Predictability and limitations of non-invasive murine tonometry: Comparison of two devices
Predictability and limitations of non-invasive murine tonometry: Comparison of two devices
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DOI：
10.1016/j.exer.2005.12.005
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发表时间：
2006-07-01
影响因子：
3.4
通讯作者：
Grosskreutz, Cynthia L.
中科院分区：
文献类型：
--
作者：
Filippopoulos, Theodoros;Matsubara, Akihisa;Grosskreutz, Cynthia L.
我们的目的是评估准确性；两种用于小鼠眼压测量的无创眼压计的可重复性和预测能力。对比了冲击回弹式眼压计（I-R）和光纤压力传感器光学干涉式眼压计（OIT）。采用C57/BL6小鼠去核眼进行标定。前房插管，以5cm H2O的增量调整IOP（开旋塞法）。为每只眼睛生成一条校准曲线，同时为所有眼睛生成一条主校准曲线。两名操作员测量了IOP。然后交替使用这些仪器测量C57/BL6和DBA2/J动物的IOP。随后将同一只眼睛插管，并确定无创眼压计的误差。两种眼压计的离体校准曲线基本相同，R-2分别为0.9878和0.9902。两种仪器的重现性都很高。在体内，I-R眼压计低估了IOP，而OIT高估了IOP。这个错误是系统性的，因此是可以预见的。该误差的置信区间是通过比较每个眼压计提供的IOP估计值与体内插管获得的有创测量值来确定的。I-R和OIT误差的95% CI分别为2.36 mmHg和2.62 mmHg。在小鼠体内进行无创血压计是可行的。这两种非侵入性仪器都提供准确和可重复的测量结果，而OIT需要每个个体的校准曲线。调查员。(c) 2006 Elsevier Ltd.版权所有。
Our purpose was to evaluate the accuracy; reproducibility and predictive ability of two non-invasive tonometers developed for intraocular pressure (IOP) measurements in the mouse. The prototype impact-rebound tonometer (I-R) and a prototype optical interferometry tonometer (OIT) utilizing a fiberoptic pressure sensor, were compared. Enucleated eyes from C57/BL6 mice were used for the calibration. The anterior chamber was cannulated and the IOP was adjusted in increments of 5 cm of H2O (open stopcock method). A calibration curve was generated for each individual eye along with a master calibration curve for all eyes. Two operators measured the IOP. The instruments were then used in alternating order to measure the IOP in C57/BL6 and in DBA2/J animals. The same eyes were subsequently cannulated and the error of the non-invasive tonometers was determined. Both tonometers yielded almost equivalent ex vivo calibration curves with individual R-2 of 0.9878 and 0.9902 respectively. Both instruments were highly reproducible. In vivo the I-R tonometer underestimated while the OIT overestimated the IOP. This error was systematic and therefore predictable. The confidence intervals of this error were determined by comparing the IOP estimates provided by each tonometer with the measurements obtained invasively by cannulation in vivo. The 95% CI of the error were 2.36 mmHg for the I-R and 2.62 mmHg for the OIT respectively. Non-invasive tonometry in the mouse is feasible. Both non-invasive instruments provide accurate and reproducible measurements with the OIT requiring calibration curves for each individual. investigator. (c) 2006 Elsevier Ltd. All rights reserved.