In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice
In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice
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DOI：
10.1016/j.jnutbio.2005.01.006
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发表时间：
2005-06-01
影响因子：
5.6
通讯作者：
Heber, D
中科院分区：
文献类型：
--
作者：
Seeram, NP;Adams, LS;Heber, D
石榴（Punica Granatum L.）水果被广泛消耗为果汁（PJ）。 PJ的有效抗氧化剂和抗动脉粥样硬化活性归因于其多酚，包括Punicalagin，主要的水果Ellagitannin和Ellagic Acid（EA）。 Punicalagin是PJ中主要的抗氧化剂多酚成分。 Punicalagin，EA，标准化的石榴单宁（TPT）提取物和PJ的体外抗增殖，凋亡和抗氧化活性。对人口服（KB，Cal27），结肠（HT-29，HCT116，SW480，SW620）和前列腺（RWPE-1，22RV1）肿瘤细胞的人口腔（KB，Cal27）和肿瘤细胞评估了Punicalagin，EA和TPT的抗增生活性，以12.5-100 mu g/ml的人类口服（KB，CAL27）（KB，Cal27）（KB，Cal27） 。以100 mu g/ml的浓度评估punicalagin，ea和tpt的凋亡作用，抗氧化特性的凋亡作用和10 mu g/ml浓度。但是，为了评估其他PJ植物化学物质的协同和/或添加剂的贡献，以标准化的浓度测试了PJ，以提供等效量的punicalagin（w/w）。评估了针对HT-29和HCT116结肠癌细胞系的凋亡作用。使用脂质过氧化和Trolox当量抗氧化能力（TEAC）测定法评估抗氧化作用。石榴汁通过抑制30％至100％的增殖表现出对所有细胞系的抗增殖活性最大。在100 mu g/ml，PJ，EA，Punicalagin和TPT诱导HT-29结肠细胞中的凋亡。然而，在HCT116结肠细胞中，EA，Punicalagin和TPT，但PJ不诱导凋亡。抗氧化活性的趋势是Pj> tpt> punicalagin> ea。与单个纯化的活性成分相比，PJ与其纯化的多酚相比，PJ的出色生物活性说明了多种化合物作用的多因素作用和化学协同作用。 （c）2005 Elsevier Inc.保留所有权利。
Pomegranate (Punica granatum L.) fruits are widely consumed as juice (PJ). The potent antioxidant and anti-atherosclerotic activities of PJ are attributed to its polyphenols including punicalagin, the major fruit ellagitannin, and ellagic acid (EA). Punicalagin is the major antioxidant polyphenol ingredient in PJ. Punicalagin, EA, a standardized total pomegranate tannin (TPT) extract and PJ were evaluated for in vitro antiproliferative, apoptotic and antioxidant activities. Punicalagin, EA and TPT were evaluated for antiproliferative activity at 12.5-100 mu g/ml on human oral (KB, CAL27), colon (HT-29, HCT116, SW480, SW620) and prostate (RWPE-1, 22Rv1) tumor cells. Punicalagin, EA and TPT were evaluated at 100 mu g/ml concentrations for apoptotic effects and at 10 mu g/ml concentrations for antioxidant properties. However, to evaluate the synergistic and/or additive contributions from other PJ phytochemicals, PJ was tested at concentrations normalized to deliver equivalent amounts of punicalagin (w/w). Apoptotic effects were evaluated against the HT-29 and HCT116 colon cancer cell lines. Antioxidant effects were evaluated using inhibition of lipid peroxidation and Trolox equivalent antioxidant capacity (TEAC) assays. Pomegranate juice showed greatest antiproliferative activity against all cell lines by inhibiting proliferation from 30% to 100%. At 100 mu g/ml, PJ, EA, punicalagin and TPT induced apoptosis in HT-29 colon cells. However, in the HCT116 colon cells, EA, punicalagin and TPT but not PJ induced apoptosis. The trend in antioxidant activity was PJ > TPT > punicalagin > EA. The superior bioactivity of PJ compared to its purified polyphenols illustrated the multifactorial effects and chemical synergy of the action of multiple compounds compared to single purified active ingredients. (c) 2005 Elsevier Inc. All rights reserved.