Large-scale expression and purification of a G-protein-coupled receptor for structure determination - an overview
Large-scale expression and purification of a G-protein-coupled receptor for structure determination - an overview
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DOI：
10.1007/s10969-005-1917-6
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发表时间：
2005-09-01
期刊：
影响因子：
--
通讯作者：
Shiloach, Joseph
中科院分区：
文献类型：
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作者：
Grisshammer, Reinhard;White, Jim F.;Shiloach, Joseph
G蛋白偶联受体的结构测定和其他应用，如核磁共振研究，需要定期获得毫克量的纯化的功能性受体蛋白。我们对神经降压素受体的表达和纯化研究进行了综述。在大肠杆菌中的功能表达和自动化的双柱纯化程序允许进行结晶实验和受体结合配体的研究。
Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance studies, require milligram quantities of purified, functional receptor protein on a regular basis. We present an overview on expression and purification studies with a receptor for neurotensin. Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound ligands.