Genetic manipulation of Agrobacterium.
Genetic manipulation of Agrobacterium.
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DOI：
10.1002/9780471729259.mc03d02s25
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发表时间：
2012-05-01
影响因子：
--
通讯作者：
Fuqua, Clay
中科院分区：
文献类型：
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作者：
Morton, Elise R;Fuqua, Clay
农杆菌是根瘤菌的植物亲缘种。一些物种会引起植物病害，如冠瘤和毛状根，尽管也有无毒物种。根癌农杆菌是研究最深入的物种，它会引起冠瘤，这是一种发生在多种植物上的肿瘤疾病。毒力是由大的质粒指定的，对于根癌农杆菌，这被称为钛(肿瘤诱导)质粒。在致病过程中，致病农杆菌复制Ti质粒的一段并将其转移到植物中，在植物中随后整合到植物基因组中，并表达导致疾病症状的基因。根癌农杆菌是一种广泛应用于植物基因工程的工具，也是一种在寄主-微生物联合、水平基因转移、细胞-细胞通讯和生物膜形成方面得到了很好研究的模式微生物。本单元描述了根癌农杆菌基因操作的标准方案。
Agrobacterium species are plant-associated relatives of the rhizobia. Several species cause plant diseases such as crown gall and hairy root, although there are also avirulent species. A. tumefaciens is the most intensively studied species and causes crown gall, a neoplastic disease that occurs on a variety of plants. Virulence is specified by large plasmids, and in the case of A. tumefaciens, this is called the Ti (tumor-inducing) plasmid. During pathogenesis virulent agrobacteria copy a segment of the Ti plasmid and transfer it to the plant, where it subsequently integrates into the plant genome, and expresses genes that result in the disease symptoms. A. tumefaciens has been used extensively as a plant genetic engineering tool and is also a model microorganism that has been well studied for host-microbe associations, horizontal gene transfer, cell-cell communication, and biofilm formation. This unit describes standard protocols for genetic manipulation of A. tumefaciens.