REVEILLE2 Thermosensitive Splicing: A Molecular Basis for the Integration of Nocturnal Temperature Information by the Arabidopsis Circadian Clock
REVEILLE2 Thermosensitive Splicing: A Molecular Basis for the Integration of Nocturnal Temperature Information by the Arabidopsis Circadian Clock
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DOI：
10.1101/2023.04.24.538045
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发表时间：
2023-04
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影响因子：
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通讯作者：
A. James;Chantal Sharples;Janet Laird;E. A. Armstrong;Wenbin Guo;Nikoleta A Tzioutziou;Runxuan Zhang;John W. S. Brown;H. G. Nimmo;M. Jones
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作者：
A. James;Chantal Sharples;Janet Laird;E. A. Armstrong;Wenbin Guo;Nikoleta A Tzioutziou;Runxuan Zhang;John W. S. Brown;H. G. Nimmo;M. Jones
低温胁迫是限制植物生长和产量的主要环境因子之一。然而，人们仍然没有完全理解植物如何解释每日的温度波动，也没有完全理解这些温度变化如何与其他调节系统（如生物钟）相结合。我们证明，REVEILLE2，MYB样转录因子，表现出冷诱导的选择性剪接开关从非翻译异构体在环境温度下的翻译异构体后，冷暴露。我们使用分子遗传学、转录组学和生理学的组合来探索REVEILLE 2的生物学功能。破坏REVEILLE2冷却开关改变调节基因的表达，损害昼夜节律的时间，并提高光合能力。核基因表达的变化在低温后的最初几个小时特别明显，随后叶绿体基因表达上调。REVEILLE2冷开关扩展了我们对植物对冷却的即时反应的理解。我们建议，昼夜节律组件REVEILLE2限制植物夜间温度降低的反应，从而使日常环境变化的适当反应。植物需要对温度做出适当的反应，考虑到相对于白天每天晚上发生的温度降低的预期日常模式。在这里，我们表明，在夜间表达的基因履行这一功能。
Cold stress is one of the major environmental factors that limit growth and yield of plants. However, it is still not fully understood how plants account for daily temperature fluctuations, nor how these temperature changes are integrated with other regulatory systems such as the circadian clock. We demonstrate that REVEILLE2, a MYB-like transcription factor, exhibits a cold-induced alternative splicing switch from a non-translatable isoform at ambient temperature to a translatable isoform upon cold exposure. We explore the biological function of REVEILLE2 using a combination of molecular genetics, transcriptomics, and physiology. Disruption of the REVEILLE2 cooling switch alters regulatory gene expression, impairs circadian timing, and improves photosynthetic capacity. Changes in nuclear gene expression are particularly apparent in the initial hours following chilling, with chloroplast gene expression subsequently up-regulated. The REVEILLE2 cold switch extends our understanding of plants immediate response to cooling. We propose that the circadian component REVEILLE2 restricts plants responses to nocturnal reductions in temperature, thereby enabling appropriate responses to daily environmental changes. Plain language summary Plants need to respond appropriately to temperature, accounting for the expected daily patterns of reduced temperatures that occur every night relative to the day. Here, we show that a gene expressed at night fulfils this function.