EXPRESSION OF HUMAN CARDIAC ACTIN IN MOUSE L-CELLS - A SARCOMERIC ACTIN ASSOCIATES WITH A NONMUSCLE CYTOSKELETON
EXPRESSION OF HUMAN CARDIAC ACTIN IN MOUSE L-CELLS - A SARCOMERIC ACTIN ASSOCIATES WITH A NONMUSCLE CYTOSKELETON
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DOI：
10.1016/0092-8674(84)90351-9
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
SKOULTCHI, AI
中科院分区：
文献类型：
--
作者：
GUNNING, P;PONTE, P;SKOULTCHI, AI
将克隆的人心脏肌动蛋白基因导入小鼠Ltk-细胞，在几种胸苷激酶（tk）阳性共转染物中表达。该克隆不仅能产生真正的聚腺苷化人心脏肌动蛋白mRNA，而且还能合成人心脏肌动蛋白。心脏肌动蛋白，通常只存在于肌原纤维中，稳定积累在高水平，约为内源性小鼠。β -肌动蛋白的1/3。此外，该肌聚蛋白在Triton X-100不溶相和可溶相之间的分裂程度与内源性- β -肌动蛋白相同。肌动蛋白显然可以参与Triton x -100不溶性细胞骨架结构的形成。
A cloned human cardiac actin gene, introduced into mouse Ltk- cells, is expressed in several thymidine kinase (tk)-positive cotransfectants. The clones not only produce authentic polyadenylated human cardiac actin mRNA but also synthesize human cardiac actin protein. The cardiac actin protein, normally found only in myofibrils, is stably accumulated at a high level, about 1/3 that of the endogenous mouse .beta.-actin. Furthermore, this sarcomeric protein partitions between the Triton X-100 insoluble and soluble phases to the same extent as the endogenous .beta.-actin. A sarcomeric actin apparently can participate in the formation of Triton X-100-insoluble cytoskeletal structures.