Analysis of Epstein Barr Virus Encoded RNA Expression in Nasopharyngeal Carcinoma in North-Eastern India: A Chromogenic in Situ Hybridization Based Study
Analysis of Epstein Barr Virus Encoded RNA Expression in Nasopharyngeal Carcinoma in North-Eastern India: A Chromogenic in Situ Hybridization Based Study
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DOI：
10.22038/ijorl.2016.7036
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发表时间：
2016-07
影响因子：
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通讯作者：
A. Saikia;V. Raphael;N. Shunyu;Y. Khonglah;J. Mishra;A. Jitani;J. Medhi
中科院分区：
文献类型：
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作者：
A. Saikia;V. Raphael;N. Shunyu;Y. Khonglah;J. Mishra;A. Jitani;J. Medhi
简介：鼻咽癌（NPC）是印度东北部地区的常见癌症。尽管在印度东北部地区 NPC 的环境贡献者的作用已被牢固确立，但 EBV 作为该地区的病原仍有待探索。材料和方法：本研究纳入 51 名在 NEIGRIHMS 耳鼻喉科就诊并经组织病理学检查证实为鼻咽癌的患者。显色原位杂交 (CISH) 用于评估 EBER（Epstein Barr 病毒编码 RNA）。核信号的存在被视为EBER表达阳性。 EBER 状态与各种临床病理参数相关，如年龄、性别、饮食习惯、鼻咽癌的组织学类型和患者的种族。结果：研究组年龄范围为25～70岁，平均年龄44.64岁，男女比例为3：2。非角化未分化型鼻咽癌是最常见的组织学类型。 59% (30/51) 的病例中 EBV 呈阳性。它与纳迦社区（P=0.01）、熏制食品消费（P=0.02）和吸烟（P=0.02）呈显着统计学相关。 EBV与年龄、性别、淋巴结转移、分期和组织学没有相关性。结论：我们的结果表明 EBV 可能是印度该地区 NPC 发病机制的另一个危险因素。因此，除了生活方式的改变之外，未来甚至可以考虑对无症状患者进行 EBV 病毒载量筛查测试，以确定疾病易感性、早期诊断和适当的治疗。
Introduction: Nasopharyngeal carcinoma (NPC) is a common cancer in the North-East region of India. Though the role of environmental contributors of NPC in the North-Eastern part of India is firmly established, EBV as an etiological agent in the region remains unexplored. Material and Methods: Fifty-one patients, who presented at the department of ENT, NEIGRIHMS and were confirmed as NPC upon histopathological examination, were included in the study. Chromogenic in-situ hybridization (CISH) was used for the evaluation of EBER (Epstein Barr Virus Encoded RNA). Presence of nuclear signals was taken as positive for EBER expression. EBER status was correlated with various clinicopathological parameters like age, sex, dietary habits, histological types of NPC, and ethnicity of the patients. Results: The age range of the study group was 25 to 70 years with a mean age of 44.64 years and a male:female ratio of 3:2. Non-keratinizing undifferentiated type of NPC was the most common histological type. EBV was positive in 59% (30/51) of our cases. It showed a statistically significant correlation with the Naga community (P=0.01), with consumption of smoked food (P=0.02), and cigarette smoking (P=0.02). There was no correlation of EBV with age, sex, lymph node metastasis, stage, and histology. Conclusion: Our result indicates that EBV may be an additional risk factor in the pathogenesis of NPC in this region of India. So apart from lifestyle modification, a future study for a screening test for EBV viral load even in asymptomatic patients may be considered, for determination of disease susceptibility, early diagnosis, and proper management.