Malondialdehyde and 4-hydroxy-2-nonenal in plant tissue cultures: LC-MS determination of 2,4-dinitrophenylhydrazone derivatives
Malondialdehyde and 4-hydroxy-2-nonenal in plant tissue cultures: LC-MS determination of 2,4-dinitrophenylhydrazone derivatives
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DOI：
10.3109/10715769709065764
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发表时间：
1997-01-01
影响因子：
3.3
通讯作者：
Benson, EE
中科院分区：
文献类型：
--
作者：
Deighton, N;Magill, WJ;Benson, EE
利用LC-MS技术，在植物组织培养物中检测到了具有细胞学活性的二次脂质过氧化产物丙二醛（MDA）和4-羟基-2-壬烯醛（HNE）的2，4-二硝基苯腙（DNP）衍生物。两种衍生物的检测限分别约为5 pmol（1.2 × 10（-9）g; 1 μ M）和0.1pmol（3 × 10（-11）g; 20 nM）。质谱响应在2-200 μ M DNP-MDA和0.02-10 μ M DNP-HNE范围内呈线性。MDA和HNE的谱可以与胚胎发生能力相关的发现是相当感兴趣的，因为氧化状态已经被牵连作为动物发育中的调节因子。
The cytologically active secondary lipid peroxidation products, malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE) have been detected as their 2,4-dinitrophenylhydrazone (DNP) derivatives in plant tissue cultures using LC-MS. This paper reports, for the first time, the use of LC-MS methodology to definitively identify 4-hydroxy-2-nonenal in plants. Limits of detection for the two derivatives are approximately 5pmol (1.2 x 10(-9)g; 1 mu M) and 0.1pmol (3 x 10(-11)g; 20nM) respectively. Mass spectrometer response was linear in the range from 2-200 mu M DNP-MDA and 0.02-10 mu M DNP-HNE.This methodology has been used to assess the formation of aldehydic secondary lipid peroxidation products in dedifferentiated callus cultures of Daucus carota. The finding that profiles of MDA and HNE can be correlated with embryogenic competence is of considerable interest as oxidative status has already been implicated as a regulatory factor in animal development.