Effects of weak static magnetic fields on the gene expression of seedlings of Arabidopsis thaliana
Effects of weak static magnetic fields on the gene expression of seedlings of Arabidopsis thaliana
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DOI：
10.1016/j.jplph.2018.08.016
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发表时间：
2018-12-01
影响因子：
4.3
通讯作者：
Galland, Paul
中科院分区：
文献类型：
--
作者：
Dhiman, Sunil K.;Galland, Paul
目前，动物和植物的磁场接收是在基于隐花色素的自由基对机制的框架下讨论的。试图解开植物磁感受的努力在历史上有几个缺点，最突出的是，明显缺乏详细的刺激-反应关系。为了确定拟南芥幼苗弱静磁场的敏感性，我们产生了近零和188亩T的基因rbcl，cab 4，pal 4和ef 1的转录水平之间的刺激响应曲线。蓝光下暗生幼苗的中等磁响应性显著增强，红光下rbcl和pal 4的中等磁响应性也显著增强。在恒定光子通量率的蓝光下获得的刺激-响应曲线显示多个最大值，因此与植物和动物生理学中普遍存在的模式根本不同。一个双突变体缺乏隐花色素1和2显示改变刺激反应曲线而不失去，但是，磁响应完全。磁场方向的逆转显著影响基因表达和CAB-蛋白（叶绿素a，b-结合蛋白）的量。我们的大多数结果与隐花色素作为唯一的磁场传感器的概念不一致。然而，他们并不排除隐花色素参与拟南芥磁场接收的可能性。研究结果有意想不到的含义，隐花色素和光敏色素介导的植物反应可以调制的强度和方向的当地地磁场。
Magnetic-field reception of animals and plants is currently discussed in the framework of a cryptochrome-based radical-pair mechanism. Efforts to unravel magnetoreception in plants suffered historically from several shortcomings, most prominently, the conspicuous absence of detailed stimulus-response relationships. To determine the sensitivity of seedlings of Arabidopsis thaliana to weak static magnetic fields we generated stimulus-response curves between near zero and 188 mu T for the transcript levels of the genes rbcl, cab4, pal4 and ef1. The moderate magneto-responsiveness of dark-grown seedlings was greatly enhanced under blue light, and for rbcl and pal4 also under red light. The stimulus-response curves obtained under blue light of constant photon-fluence rate displayed multiple maxima and thus a pattern fundamentally different from that prevalent in plant and animal physiology. A double mutant lacking cryptochromes 1 and 2 displayed altered stimulus-response curves without losing, however, magneto-responsiveness completely. A reversal of the magnetic field direction substantially affected the gene expression and the quantity of CAB-protein (chlorophyll a,b-binding protein). The majority of our results are at variance with the notion of cryptochromes acting as the only magnetic-field sensors. They do not, however, exclude the possibility that cryptochromes participate in the magnetic field reception of Arabidopsis. The findings have the unexpected implication that cryptochrome- and phytochrome-mediated plant responses can be modulated by the strength and the orientation of the local geomagnetic field.