Experimental and calculated parameters on particle phagocytosis by alveolar macrophages
Experimental and calculated parameters on particle phagocytosis by alveolar macrophages
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DOI：
10.1152/japplphysiol.01067.2001
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发表时间：
2002-06-01
影响因子：
3.3
通讯作者：
Jarstrand, C
中科院分区：
文献类型：
--
作者：
Camner, P;Lundborg, M;Jarstrand, C
三种类型的荧光素标记的测试颗粒大鼠肺泡巨噬细胞（AM）的吞噬作用进行了研究：球形二氧化硅（3.2妈妈），热杀死的白色念珠菌（3.8妈妈），和热杀死的新型隐球菌（6.1妈妈）调理与特定的IgG。这些颗粒应分别附着于清道夫、甘露糖和Fc受体。研究了对照AM和用干扰素-γ（12.5或50 U/ml）预处理20 h的AM。将每个AM的附着和摄入颗粒数的总和（累积附着）用作附着过程的量度，并且将每个AM的摄入颗粒数除以累积附着（摄入分数）用作摄入过程的量度。平均摄入时间（IT），这也是一个摄入过程的措施，从实验数据计算。摄取过程独立于附着过程。随着观察时间的延长，其增加。这是因为，由短于特定粒子的IT的整个分布的观测时间确定的IT导致比真实的平均IT更短的IT。白色念珠菌（甘露糖受体）的摄食过程最快，C.用特异性IgG（Fc受体）调理的新生儿具有几乎同样快的摄取，而二氧化硅颗粒（清道夫受体）具有最慢的摄取过程。用干扰素-γ治疗显著损害了所有三种类型的颗粒（和三种类型的受体）的附着过程，但明显损害了仅二氧化硅颗粒（清道夫受体）的摄取过程。
Phagocytosis of three types of fluorescein-labeled test particles by rat alveolar macrophages (AM) were studied: spherical silica (3.2 mum), heat-killed Candida albicans (3.8 mum), and heat-killed Cryptococcus neoformans (6.1 mum) opsonized with specific IgG. These particles should attach to scavenger, mannose, and Fc receptors, respectively. Both control AM and AM pretreated for 20 h with interferon-gamma (12.5 or 50 U/ml) were studied. The sum of the number of attached and ingested particles per AM (accumulated attachment) was used as a measure of the attachment process, and the number of ingested particles per AM divided by the accumulated attachment (ingested fraction) was used as a measure of the ingestion process. The average ingestion time (IT), which is also a measure of the ingestion process, was calculated from the experimental data. The ingestion process was independent of the attachment process. IT increased with the time of observation. This is explained by the fact that IT determined from observation times shorter than the whole distribution of IT for a certain particle results in a shorter IT than the real average IT. C. albicans (mannose receptor) had the fastest ingestion process, C. neoformans opsonized with specific IgG (Fc receptor) had ingestion that was nearly as fast, and the silica particles (scavenger receptors) had the slowest ingestion process. Treatment with interferon-gamma markedly impaired the attachment process for all three types of particles (and three types of receptors) but clearly impaired the ingestion process only for silica particles (scavenger receptors).