Investigation of musashi-1 expressing cells in the murine model of dextran sodium sulfate-induced colitis
Investigation of musashi-1 expressing cells in the murine model of dextran sodium sulfate-induced colitis
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DOI：
10.1007/s10620-006-8046-3
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发表时间：
2006-07-01
影响因子：
3.1
通讯作者：
Kawata, Sumio
中科院分区：
文献类型：
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作者：
Fukui, Tadahisa;Takeda, Hiroaki;Kawata, Sumio
Musashi-1（Msi-1）是一种RNA结合蛋白，被认为是神经干/前体细胞的特异性标志物。肠上皮中表达Msi-1的细胞也被强烈认为是潜在的干细胞/前体细胞。为了阐明这些细胞在损伤或再生阶段的行为，我们研究了右旋糖酐硫酸钠（DSS）诱导的结肠炎小鼠模型中表达Msi-1的肠粘膜细胞。免疫组化结果显示，Msi-1阳性细胞分布于小肠Paneth细胞层的沿着和大肠隐窝的底层。DSS给药期间，大肠中PCNA阳性细胞的数量显著增加。与此相反，Msi-1阳性细胞的数量随着DSS的使用而略微减少，但在DSS给药停止后恢复正常。Msi-1 mRNA表达水平与免疫组化结果一致。总之，我们可以用Msi-1描述炎症过程中肠干细胞/前体细胞的行为。
Musashi-1 (Msi-1), an RNA-binding protein, had been proposed to be a specific marker for neural stem/precursor cells. Msi-1 expressing cells in the intestinal epithelium are also strongly considered as potential stem/precursor cells. To clarify the behavior of those cells in the injury or regeneration phase, we investigated Msi-1 expressing cells of intestinal mucosa in the murine model of dextran sodium sulfate (DSS)-induced colitis. Immunohistochemically, Msi-1-positive cells were found in the area just along the layer of Paneth's cells in the small intestine and in the bottom layer of crypts in the large intestine. During DSS administration, the number of PCNA-positive cells in the large intestine increased markedly. In contrast, the number of Msi-1-positive cells decreased slightly with DSS but returned to normal after DSS administration was stopped. The level of mRNA for Msi-1 was consistent with the result of immunohistochemical examinations. Conclusively, we could describe the behavior of intestinal stem/precursor cells during inflammation using Msi-1.