Dp71, utrophin and β-dystroglycan expression and distribution in PC12/L6 cell cocultures
Dp71, utrophin and β-dystroglycan expression and distribution in PC12/L6 cell cocultures
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DOI：
10.1097/wnr.0b013e3282f0e42d
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发表时间：
2007-10-29
期刊：
影响因子：
1.7
通讯作者：
Montanez, Cecilia
中科院分区：
文献类型：
--
作者：
Ilarraza-Lomeli, Ramses;Cisneros-Vega, Bulmaro;Montanez, Cecilia
抗肌萎缩蛋白Dp 71亚型的功能尚不清楚，但似乎与神经突生长和突触形成有关。为了评估Dp 71在肌神经突触中的作用，我们使用PC 12细胞和L 6肌管建立了共培养模型，并分析了Dp 71和相关蛋白utrophin和P-dystroglycan在PC 12细胞中的表达和定位。共聚焦显微镜显示，Dp 71 d亚型在PC 12细胞核，高尔基复合体样和内质网样结构，而Dp 71 ab集中在轴突尖端和细胞质，共定位与β-肌营养不良蛋白聚糖，utrophin，突触素和乙酰胆碱受体。有证据表明，Dp 71 ab亚型，不像Dp 71 d，可能参与神经突相关的过程。这是第一个工作的Dp和成员的Dip相关蛋白复合物的作用，在细胞系为基础的共培养系统，这可能是有用的，在确定Dp 71亚型协会。(C)2007年威科健康垂直酒吧利平科特威廉姆斯&威尔金斯。
Function of dystrophin Dp71 isoforms is unknown but seems related to neurite outgrowth and synapse formation. To evaluate Dp71 role in myoneural synapses, we established a coculture model using PC12 cells and L6 myotubes and analyzed expression and localization of Dp71 and related proteins, utrophin and P-dystroglycan, in PC12 cells. Confocal microscopy showed Dp71d isoform in PC12 nuclei, golgi-complex-like and endoplasmic reticulum-like structures, whereas Dp71ab concentrates at neurite tips and cytoplasm, colocalizing with beta-dystroglycan, utrophin, synaptophysin and acetylcholine receptors. Evidences suggest that Dp71ab isoform, unlike Dp71d, may take part in neurite-related processes. This is the first work on Dp and members of Dip-associated protein complex roles in a cell-line based coculturing system, which may be useful in determining Dp71 isoforms associations. (C) 2007 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.