Expression of keratinocyte growth factor and its receptor in rat tracheal cartilage: possible involvement in wound healing of the damaged cartilage.
Expression of keratinocyte growth factor and its receptor in rat tracheal cartilage: possible involvement in wound healing of the damaged cartilage.
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DOI：
10.1267/ahc.10006
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发表时间：
2010-06-28
影响因子：
2.4
通讯作者：
Koji T
中科院分区：
文献类型：
--
作者：
Abo T;Nagayasu T;Hishikawa Y;Tagawa T;Nanashima A;Yamayoshi T;Matsumoto K;An S;Koji T
角质细胞生长因子（KGF）参与多种组织的发育和再生。为了阐明KGF在软骨创面愈合中的作用，我们通过免疫组化方法检测了KGF及其受体（KGFR）在大鼠气管软骨创面愈合区的表达，以及重组KGF对大鼠软骨细胞原代培养增殖和分化的直接影响。软骨细胞和软骨膜外细胞的细胞质中均发现KGF。另一方面，KGFR仅在软骨细胞的质膜中检测到。虽然损伤前后软骨和软骨膜区的KGF表达相似，但损伤后KGFR表达被诱导，且仅限于增殖的软骨细胞。成熟和未成熟大鼠气管软骨中KGF和KGFR的染色模式相同。此外，用原代培养的软骨细胞进行的体外实验显示，200 ng/ml的KGF显著增加软骨细胞数量（约1.5倍），并显著减少酸性粘多糖的产生。这些结果表明，KGF刺激软骨细胞增殖，提示KGF可以治疗性地调节气管软骨的伤口愈合过程。
Keratinocyte growth factor (KGF) is involved in the development and regeneration of a variety of tissues. To clarify the role of KGF in cartilage wound healing, we examined the expression of KGF and its receptor (KGFR) immunohistochemically in the wound healing area of rat tracheal cartilage, and the direct effect of recombinant KGF on the proliferation and differentiation of primary cultures of rat chondrocytes. KGF was found in the cytoplasm of both chondrocytes and perichondrial cells. On the other hand, KGFR was detected only in the plasma membrane of chondrocytes. Although the expression of KGF was similar in the cartilage and perichondrial area before and after injury, KGFR expression was induced after injury and limited to proliferating chondrocytes. The staining pattern of KGF and KGFR was same in the mature and the immature rat tracheal cartilage. Moreover, in vitro experiments using primary cultured chondrocytes revealed that KGF at 200 ng/ml significantly increased the number of chondrocytes (~1.5-fold), and significantly reduced acid mucopolysaccharide production. These results indicate that KGF stimulates chondrocyte proliferation, suggesting that KGF could therapeutically modulate the wound healing process in the tracheal cartilage.