Functional characterization of a protein inhibitor of activated STAT (PIAS) gene in Litopenaeus vannamei
Functional characterization of a protein inhibitor of activated STAT (PIAS) gene in Litopenaeus vannamei
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凡纳滨对虾激活 STAT (PIAS) 基因蛋白抑制剂的功能表征
DOI：
10.1016/j.fsi.2019.09.007
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发表时间：
2019
影响因子：
4.7
通讯作者：
Shi Lili
中科院分区：
文献类型：
--
作者：
Zhang Shuang;Li Chaozheng;Wang Wei;Wang Chenggui;Sun Chengbo;Chan Siuming;Shi Lili
激活的STAT蛋白抑制剂（皮亚斯）在哺乳动物和果蝇中作为负调节因子在JAK/STAT信号通路的反馈调节中起着关键作用，但皮亚斯在甲壳动物中的功能尚不清楚。本研究从凡纳滨对虾（Litopenaeus vannamei）中克隆了一个皮亚斯（PIAS）基因，命名为LvPIAS。LvPIAS基因全长3065 bp，其中开放阅读框（ORF）2361 bp，编码786个氨基酸，在肌肉中表达量最高，能抵抗LPS、副溶血性弧菌、金黄色葡萄球菌、Poly I：C和白色斑点综合征病毒（WSSV）的攻击。LvPIAS可被转录因子LvSTAT诱导，但LvPIAS可抑制LvSTAT对LvPIAS启动子的转录活性，说明LvSTAT与LvPIAS之间存在负反馈环。此外，RNAi介导的LvPIAS基因敲减组对虾对WSSV感染的存活率高于对照组（dsGFP注射组），提示LvPIAS基因可能对WSSV感染起负作用。
Protein inhibitor of activated STAT (PIAS) plays a critical role in the feedback modulation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway as a negative regulator in mammals andDrosophila, but the function of PIAS in crustaceans is still unclear. In this study, a PIAS termedLvPIASwas cloned and characterized from Litopenaeus vannamei. The full length ofLvPIASwas 3065 bp, including a 2361 bp open reading frame (ORF) coding for a protein of 786 aa.LvPIASexpression was most abundant in muscle and could respond to the challenge of LPS, Vibrio parahaemolyticus,Staphhylococcus aureus, Poly I: C and white spot syndrome virus (WSSV). LvPIAS could be induced by the transcription factor LvSTAT, but LvPIAS could inhibit the transcriptional activity of LvSTAT to the LvPIAS promoter conversely, which indicated that there was a negative feedback loop between LvSTAT and LvPIAS. Furthermore, RNAi-mediated knockdown ofLvPIASshrimps showed higher survival rate to WSSV infection than those in the control group (dsGFP injection), suggesting that LvPIAS may play a negatively role against WSSV infection.