Disruption of the Gene trx-m1 Impedes the Growth of Anabaena sp. PCC 7120 under Nitrogen Starvation
Disruption of the Gene trx-m1 Impedes the Growth of Anabaena sp. PCC 7120 under Nitrogen Starvation
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DOI：
10.1093/pcp/pcz056
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发表时间：
2019-07-01
影响因子：
4.9
通讯作者：
Hisabori, Toru
中科院分区：
文献类型：
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作者：
Deschoenmaeker, Frederic;Mihara, Shoko;Hisabori, Toru
蓝藻具有复杂的光合作用为基础的代谢与令人钦佩的可塑性。这种可塑性是可能的，通过深调节网络，巯基氧化还原调节操作的硫氧还蛋白（以下简称Trx）。在这种情况下，我们表征了鱼腥藻的Trx-m1缺陷突变株，PCC 7120（简称A.7120），在氮限制下培养。Trx-m1似乎协调氮的反应，其缺乏诱导蛋白质组的大的变化。我们的数据清楚地表明，Trx-m1是至关重要的固氮生长的A.7120。Trx-m1的缺乏导致异形胞的大分化（>总细胞的20%），其几乎没有功能，可能是由于固氮酶的弱表达。此外，突变株的异形胞没有显示通常的固氮细胞的细胞结构。这揭示了为什么突变株不能在氮饥饿下生长。
Cyanobacteria possess a sophisticated photosynthesis-based metabolism with admirable plasticity. This plasticity is possible via the deep regulation network, the thiol-redox regulations operated by thioredoxin (hereafter, Trx). In this context, we characterized the Trx-m1-deficient mutant strain of Anabaena sp., PCC 7120 (shortly named A.7120), cultivated under nitrogen limitation. Trx-m1 appears to coordinate the nitrogen response and its absence induces large changes in the proteome. Our data clearly indicate that Trx-m1 is crucial for the diazotrophic growth of A.7120. The lack of Trx-m1 resulted in a large differentiation of heterocysts (> 20% of total cells), which were barely functional probably due to a weak expression of nitrogenase. In addition, heterocysts of the mutant strain did not display the usual cellular structure of nitrogen-fixative cells. This unveiled why the mutant strain was not able to grow under nitrogen starvation.