Over-expression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants
Over-expression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants
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DOI：
10.1093/pcp/pcg167
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发表时间：
2003-12-01
影响因子：
4.9
通讯作者：
Kasamo, K
中科院分区：
文献类型：
--
作者：
Katsuhara, M;Koshio, K;Kasamo, K
大麦HvPIP 2; 1是质膜水通道蛋白，并且其表达在大麦中盐胁迫后下调[Katsuhara等人（2002）Plant Cell Physiol.43：885]。本研究中，我们生产并分析了过表达大麦HvPIP 2;1的转基因水稻植株。HvPIP 2;1的过表达使（1）根的径向导水率（Lp（r））增加到140%，（2）地上部与根的质量比增加到150%。在100 mM NaCl的盐胁迫下，这些转基因水稻植株的生长减少大于非转基因植株。盐胁迫2周后，转基因植株地上部含水量下降（从79%降至61%），根质量或地上部质量下降（均低于非胁迫植株的40%）。这些结果表明，HvPIP 2;1的过表达使水稻植株对100 mM NaCl敏感。水通道蛋白可能参与耐盐性进行了讨论。
Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Overexpression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lp(r)) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.