FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxyl-terminal domain of RNA polymerase IIO
FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxyl-terminal domain of RNA polymerase IIO
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DOI：
10.1074/jbc.273.42.27593
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发表时间：
1998-10-16
影响因子：
4.8
通讯作者：
Greenblatt, J
中科院分区：
文献类型：
--
作者：
Archambault, J;Pan, GH;Greenblatt, J
TFIIF （RAP30/74）是一种通用的起始因子，也可以提高RNA聚合酶II的延伸率。RAP74相互作用蛋白的双杂交筛选产生编码FCP1a的cdna， FCP1a是一种新的，普遍表达的人类蛋白，与RAP74的羧基末端进化保守结构域相互作用。编码FCP1b的相关cdna缺乏FCP1a的羧基末端rap74结合结构域，FCP1是rap74刺激的磷酸酶的一个重要亚基，该酶会对最大的RNA聚合酶II亚基的羧基末端结构域进行持续去磷酸化，FCP1也是人类RNA聚合酶II全酶复合物的一个化学计量成分。
TFIIF (RAP30/74) is a general initiation factor that also increases the rate of elongation by RNA polymerase II. A two-hybrid screen for RAP74 interacting proteins produced cDNAs encoding FCP1a, a novel, ubiquitously expressed human protein that interacts with the carboxyl-terminal evolutionarily conserved domain of RAP74. Related cDNAs encoding FCP1b lack a carboxyl-terminal RAP74-binding domain of FCP1a, FCP1 is an essential subunit of a RAP74-stimulated phosphatase that processively dephosphorylates the carboxyl-terminal domain of the largest RNA polymerase II subunit, FCP1 is also a stoichiometric component of a human RNA polymerase II holoenzyme complex.