Influence of Sampling Conditions, Salivary Flow, and Total Protein Content in Uric Acid Measurements in Saliva
Influence of Sampling Conditions, Salivary Flow, and Total Protein Content in Uric Acid Measurements in Saliva
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DOI：
10.3390/antiox8090389
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发表时间：
2019-09-01
期刊：
影响因子：
7
通讯作者：
Tvarijonaviciute, Asta
中科院分区：
文献类型：
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作者：
Gonzalez-Hernandez, Jorge M.;Franco, Lorena;Tvarijonaviciute, Asta
尿酸（UA）是唾液中含量最丰富的抗氧化化合物，也是检测机体氧化状态变化最敏感的生物标志物之一。本研究的目的是评估：（i）不同的唾液采集方法以及（ii）通过唾液流量或总蛋白校正对唾液中尿酸浓度的影响。12名健康男性在进行两种不同强度的抗阻训练（这两种训练会使唾液中尿酸浓度产生差异）后，分别采集配对的唾液（通过两种不同方法采集，即被动流涎法和使用Salivette棉卷法）和血清样本。使用Salivette棉卷法和被动流涎法采集的唾液中尿酸值之间没有显著差异。当通过被动流涎法和使用Salivette棉卷法采集唾液样本且未通过唾液流量或总蛋白浓度进行校正时，检测到血清和唾液中尿酸之间的相关性以及运动后唾液中尿酸的增加。因此，对于唾液中尿酸的测量，不建议通过唾液流量或蛋白浓度对结果进行标准化。本研究强调了在唾液中测量分析物时选择合适的采样方法以及结果表达的重要性。
Uric acid (UA) is the most abundant antioxidant compound in saliva and one of the most sensitive biomarkers for detecting changes in the oxidative status of the organism. The aim of this study was to evaluate the effect of: (i) different methods of saliva sampling and (ii) the correction by salivary flow or total protein on UA concentrations in saliva. Paired saliva (collected by two different methods, passive drooling and using Salivette cotton rolls) and serum samples were obtained from 12 healthy men after the performance of two resistance training exercises of different level of effort that can produce different concentrations in UA in saliva. There were no significant differences between values of uric acid in saliva using Salivette and passive drool. Correlations between UA in serum and saliva and increases in UA in saliva after exercise were detected when saliva samples were obtained by passive drool and Salivette and were not corrected by salivary flow or total protein concentration. Therefore for UA measurements in saliva it would not be recommended to normalize the results by salivary flow or protein concentration. This study highlights the importance of choosing an adequate sampling method selection as well as the expression of results when analytes are measured in saliva.