Ultrastructure of enucleated mammalian cells in culture.
Ultrastructure of enucleated mammalian cells in culture.
复制标题
培养中去核哺乳动物细胞的超微结构。
DOI：
10.1016/0014-4827(73)90111-0
复制
发表时间：
1973
影响因子：
3.7
通讯作者：
D. Prescott
中科院分区：
文献类型：
--
作者：
G. Wise;D. Prescott
在细胞松弛素B中离心去核后30 min和24 h，描述L细胞的精细结构。30分钟去核细胞的形态与有核细胞的细胞质相同。去核细胞中有中心粒、正常的高尔基体、内质网、线粒体、微管和一些微丝。去核后24 h，去核体广泛空泡化。高尔基体池极度扩张，颗粒状内质网有时扩张。微管，特别是微丝，仍然很丰富。在细胞松弛素B中，通过去核作用从细胞中取出的细胞核被含有大量核糖体的细胞质薄壳、偶尔的胞核、几片内质网和封闭的质膜所包围。核膜和内质网之间的连续性特别频繁。这些核具有正常的精细结构。
The fine structure of L cells is described at 30 min and 24 h after enucleation by centrifugation in cytochalasin B. The morphology of the 30-min enucleates is the same as that of the cytoplasm of nucleated cells. Centrioles, a normal Golgi apparatus, endoplasmic reticulum, mitochondria, microtubules, and some microfilaments, are present in enucleates. At 24 h after enucleation, the enucleates are extensively vacuolated. The cisternae of the Golgi apparatus are extremely dilated, and the granular ER is sometimes dilated. Microtubules, and, in particular, microfilaments, are still abundant. Nuclei removed from cells by enucleation in cytochalasin B are surrounded by a thin shell of cytoplasm containing numerous ribosomes, an occasional mitochondrion, a few pieces of endoplasmic reticulum, and an enclosing plasma membrane. Continuities between the nuclear envelope and the ER are particularly frequent. These nuclei possesses a normal fine structure.