Mechanical waves during tissue expansion
Mechanical waves during tissue expansion
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DOI：
10.1038/nphys2355
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发表时间：
2012-08-01
期刊：
影响因子：
19.6
通讯作者：
Trepat, Xavier
中科院分区：
文献类型：
--
作者：
Serra-Picamal, Xavier;Conte, Vito;Trepat, Xavier
生物体形成其形状和治愈伤口的过程涉及单层细胞的扩张。尽管已知上皮扩张失败会导致多种疾病，包括癌症（约占所有人类癌症的 90%），但支撑这种上皮扩张的机制仍不清楚。在这里，使用微图案上皮单层作为模型系统，我们报告了机械波的发现，该机械波缓慢传播跨越单层，以协作方式穿过细胞间连接并建立机械应力的差异。这种波的产生和传播的基本特征是通过基于细胞骨架强化和流化的顺序前沿的最小模型来捕获的。这些发现建立了单层扩张过程中远程细胞引导、对称性破坏和模式形成的机制。
The processes by which an organism develops its shape and heals wounds involve expansion of a monolayer sheet of cells. The mechanism underpinning this epithelial expansion remains obscure, despite the fact that its failure is known to contribute to several diseases, including carcinomas, which account for about 90% of all human cancers. Here, using the micropatterned epithelial monolayer as a model system, we report the discovery of a mechanical wave that propagates slowly to span the monolayer, traverses intercellular junctions in a cooperative manner and builds up differentials of mechanical stress. Essential features of this wave generation and propagation are captured by a minimal model based on sequential fronts of cytoskeletal reinforcement and fluidization. These findings establish a mechanism of long-range cell guidance, symmetry breaking and pattern formation during monolayer expansion.