Ratiometric-enhanced G-Quadruplex Probes for Amplified and Mix-to-Read Detection of Mercury Pollution in Aquatic Products.
Ratiometric-enhanced G-Quadruplex Probes for Amplified and Mix-to-Read Detection of Mercury Pollution in Aquatic Products.
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DOI：
10.1021/acs.jafc.0c05658
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发表时间：
2020-10
影响因子：
6.1
通讯作者：
Yanping Wu;Yuxi Yue;Sha Deng;Guiping He;Hong Gao;Mi Zhou;Kai Zhong;Ruijie Deng
中科院分区：
文献类型：
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作者：
Yanping Wu;Yuxi Yue;Sha Deng;Guiping He;Hong Gao;Mi Zhou;Kai Zhong;Ruijie Deng
汞作为一种全球性有毒污染物，极易在水产品中积累，对人体健康构成极大威胁。在这项工作中，我们提出了一种混合-读取、无标签、可靠的方法，通过设计比例增强的g -四重探针来检测水产品中的汞污染。从g -四重体到发夹状结构的转变使我们能够赋予对Hg2+的比率和杠杆响应，放大Hg2+检测的信本比。通过筛选平行和反平行、单链和多链g -四链结构，进一步提高了Hg2+的响应。与普通适体探针相比，比例增强的g -四重探针对Hg2+的检测灵敏度提高了4.7倍。该传感系统允许在室温下使用单个未标记的DNA序列对Hg2+进行简单的单管均匀检测。将其应用于鱼虾中Hg2+的检测，回收率为98.5 ~ 105.9%。无标签和混合读取法有望现场检测汞污染，促进水产品的食品安全。
Mercury, as a global toxic pollutant, is easy to be accumulated in aquatic products and poses a great threat to human health. In this work, we proposed a mix-to-read, label-free, and robust assay for detecting mercury pollution in aquatic products by engineering a ratiometric-enhanced G-quadruplex probe. The transformation from the G-quadruplex to a hairpin-like structure allows us to confer a ratiometric and leveraged response to Hg2+, amplifying the signal-to-background ratio for Hg2+ detection. Hg2+ response was further improved by screening parallel- and antiparallel-, single-, and multiple-stranded G-quadruplex structures. Compared to the common aptamer probes, the ratiometric-enhanced G-quadruplex probe increased the sensitivity for Hg2+ detection by 4.7 times. This proposed sensing system allowed a simple and one-tube homogenous detection of Hg2+ at room temperature using a single unlabeled DNA sequence. Its application for Hg2+ detection in fish and shrimp conferred satisfactory recovery rates ranging from 98.5 to 105.9%. The label-free and mix-to-read assay is promising for the onsite detection of mercury pollution and facilitating food safety of aquatic products.