Nucleotide Sequences of Genes Encoding Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces

Nucleotide Sequences of Genes Encoding Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces
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编码产异糖酰胺链霉菌产生的异糖酰胺敏感和不敏感几丁质酶的基因的核苷酸序列

DOI:
10.1271/bbb.67.2002
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发表时间:
2003
期刊:
Bioscience, Biotechnology, and Biochemistry
影响因子:
--
通讯作者:
S. Sakuda
S. Sakuda
中科院分区:
--
文献类型:
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作者:
H. Matsuura;S. Okamoto;S. Anamnart;Qiuqi Wang;Zeyang Zhou;T. Nihira;Y. Yamada;T. Kuzuyama;H. Seto;J. Nakayama;A. Suzuki;H. Nagasawa;S. Sakuda

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Allosamidin是家族18几丁质酶的强抑制剂。我们以前报道的存在下,allosamidin敏感和不敏感的几丁质酶(几丁质酶S和IS)的培养滤液的allosamidin生产菌株,链霉菌属AJ9463。本研究对这两种几丁质酶的编码基因进行了克隆和序列测定,明确了几丁质酶S和IS分别属于第18和第19几丁质酶家族。
Allosamidin is a strong inhibitor of family 18 chitinases. We previously reported the presence of allosamidin-sensitive and -insensitive chitinases (chitinase S and IS) in the culture filtrate of the allosamidin-producing strain, Streptomyces sp. AJ9463. In this study, we cloned and sequenced the genes encoding the two chitinases, which clarified that chitinase S and IS belong to the family 18 and 19 chitinase, respectively.