AMPLIFIED MET GENE LINKED TO DOUBLE MINUTES IN HUMAN GLIOBLASTOMA
AMPLIFIED MET GENE LINKED TO DOUBLE MINUTES IN HUMAN GLIOBLASTOMA
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DOI：
10.1016/0959-8049(93)90460-w
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发表时间：
1993-01-01
影响因子：
8.4
通讯作者：
MEESE, E
中科院分区：
文献类型：
--
作者：
WULLICH, B;MULLER, HW;MEESE, E
发现met原癌基因在从多形性胶质母细胞瘤WHO IV级建立的人胶质母细胞瘤细胞系（T3095）中扩增。在T3095中未观察到先前在胶质母细胞瘤中描述的表皮生长因子受体、转化生长因子α和N-myc的扩增。然而，存在8倍的met扩增。Giemsa染色的T3095细胞中期显示大多数细胞中有多个（>5）双分钟（dmins）。裸鼠异种移植后，dmins的数量和频率显著增加。dmins的增加与met扩增水平（50倍）相关，表明扩增的met定位在dmins上。在这里，我们报告的第一例甲基转移酶扩增胶质母细胞瘤。met扩增与染色体外元件（dmins）之间的相关性以前未见报道。
The met proto-oncogene was found to be amplified in a human glioblastoma cell line (T3095) established from a glioblastoma multiform WHO grade IV. Amplification of epidermal growth factor receptor, transforming growth factor alpha and N-myc which have been described previously in glioblastoma were not observed in T3095. There was, however, an 8-fold met amplification. Giemsa-stained metaphases of T3095 cells revealed multiple (>5) double minutes (dmins) in the majority of cells. Following xenografting in nude mice there was a significant increase in the number and frequency of dmins. The increase in dmins correlates with the level of met amplification (50-fold), suggesting localisation of the amplified met on dmins. Here we report the first case of met amplification in glioblastoma. Correlation between met amplification and extrachromosomal elements (dmins) has not been reported previously.