Luminescence energy transfer detection of PSA in red region based on Mn2+-enhanced NaYF4:Yb, Er upconversion nanorods.
Luminescence energy transfer detection of PSA in red region based on Mn2+-enhanced NaYF4:Yb, Er upconversion nanorods.
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DOI：
10.1016/j.bios.2015.05.024
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发表时间：
2015-10
影响因子：
12.6
通讯作者：
Jianguo Zhang;Shaozhen Wang;Ni Gao;Dexiang Feng;Lun Wang;Hongqi Chen
中科院分区：
文献类型：
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作者：
Jianguo Zhang;Shaozhen Wang;Ni Gao;Dexiang Feng;Lun Wang;Hongqi Chen
设计了一种新的开启发光能量转移（LET）系统，用于检测前列腺特异性抗原（PSA，一种癌症标记物），该系统利用Mn2+增强长波长发光NaYF4:Yb、Er上转换纳米棒作为供体，金纳米棒作为受体。合成了发射峰位于红色区域的Mn2+掺杂NaYF4:Yb,Er上转换发光纳米棒。与 NaYF4:Yb、Er 上转换纳米材料（由 980 nm 连续波长激光激发）相比，Mn2+ 的存在显着增加了发光强度。用聚丙烯酸对Mn2+掺杂的NaYF4:Yb、Er上转换纳米棒的表面进行修饰。针对前列腺特异性抗原的抗体与羧基功能化上转换纳米棒的表面结合，该纳米棒在该 LET 系统中充当能量供体。通过种子生长法合成了吸收带为~666 nm 的金纳米棒，作为能量受体。上转换纳米棒的发射带与金纳米棒的吸收带很好地重叠。由于静电相互作用缩短了供体（带负电）和受体（带正电）之间的距离，发光被猝灭。当将PSA抗原添加到系统中时，由于PSA和抗PSA之间的结合亲和力大于静电相互作用，能量受体和能量供体分离，从而恢复发光。检测 PSA 的线性范围为 0.1172 至 18.75 ng/mL（R=0.995），PSA 的检测限低至 0.1129 ng/mL。该方法成功应用于人血清样品中PSA的检测。
A new turn-on luminescence energy transfer (LET) system has been designed for the detection of prostate specific antigen (PSA, a cancer marker) that utilizes Mn2+-enhanced long wavelength luminescence NaYF4:Yb, Er upconversion nanorods as the donor and gold nanorods as the acceptor. The Mn2+-doped NaYF4:Yb,Er upconversion luminescence nanorods with an emission peak located in the red region were synthesized. The presence of Mn2+markedly increased the luminescence intensity over that of the NaYF4:Yb, Er upconversion nanomaterials (excited by a 980 nm continuous wavelength laser). The surfaces of Mn2+-doped NaYF4:Yb, Er upconversion nanorods were modified with poly(acrylic acid). Antibodies against prostate specific antigen were bound to the surface of the carboxyl-functionalized upconversion nanorods, which acted as the energy donor in this LET system. Gold nanorods with an absorption band at ~666 nm were synthesized by the seed growth method, acted as the energy acceptor. The emission band of the upconversion nanorods overlapped well with the absorption band of the gold nanorods. The luminescence was quenched because of the electrostatic interactions that shortened the distance between the donor (negatively charged) and the accepter (positively charged).When the PSA antigen was added into the system, the energy acceptor and the energy donors were separated because the binding affinity between PSA and anti-PSA was greater than the electrostatic interactions, and thereby the luminescence was recovered. The linear range of detecting PSA was from 0.1172 to 18.75 ng/mL (R=0.995), with a limit of detection for PSA as low as 0.1129 ng/mL. The method was successfully applied to the sensing of PSA in human serum samples.