Degradation of caffeine by Pseudomonas monteilii KRM9
Degradation of caffeine by Pseudomonas monteilii KRM9
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蒙氏假单胞菌 KRM9 降解咖啡因
DOI：
10.21161/mjm.110217
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发表时间：
2018
影响因子：
0.3
通讯作者：
S. Suharjono
中科院分区：
文献类型：
--
作者：
S. Arimurti;T. Ardyati;Y. Nurani;T. A. Siswoyo;S. Suharjono
目的：本研究的目的是从印度尼西亚的咖啡渣废料中分离出咖啡因降解细菌，并表征其咖啡因降解活性。方法与结果：从小粒咖啡和C.内孔这些分离株是根据其咖啡因降解活性进行选择的。通过16 S rDNA序列分析和Microbact试剂盒对最佳菌株进行鉴定和生化特性分析。同时，利用LC-MS/MS分析了该菌株对咖啡因的降解活性，结果表明，有14株细菌具有降解咖啡因的能力。在咖啡因培养基上，菌株KRM 9的咖啡因降解率最高，培养24 h后达到99.26 ± 0.01%。根据16 S rDNA序列分析，初步鉴定KRM 9菌株为蒙氏假单胞菌（Pseudomonas monteilii）。到目前为止，该物种还没有被报道为咖啡因降解细菌。LC-MS/MS分析表明，咖啡因被P. monteilii KRM 9降解，可可碱不是咖啡因降解的次级代谢产物。结论、研究意义及影响：蒙氏假单胞菌KRM 9是一株新的咖啡因降解菌。这种细菌可以作为一种试剂引入，以降解咖啡渣中的咖啡因。因此，对P. monteilii KRM 9降解咖啡因的机制进行深入研究是有意义的。
Aims: The objective of this research was to isolate caffeine-degrading bacteria from coffee pulp waste in Indonesia and characterize their caffeine degradation activity. Methodology and results: The caffeine-degrading bacteria were isolated from coffee pulp wastes of Coffea arabica and C. canephora. These isolates were selected based on their caffeine degradation activity. The identification and biochemical properties of the best isolate were conducted via 16S rDNA sequence analyses and by using the Microbact kit. Meanwhile, caffeine degradation activity of this bacteria was analyzed by using LC-MS/MS. The results indicated that fourteen bacterial isolates were able to degrade caffeine. The highest caffeine degradation activity was performed by isolate KRM9 at the rate of 99.26 ± 0.01%, on a caffeine medium after 24 h of incubation. Based on the 16S rDNA analyses, the KRM9 isolate was identified as Pseudomonas monteilii. Till present, this species has not been reported as a caffeine-degrading bacterium. However, LC-MS/MS analysis indicated that caffeine was degraded by P. monteilii KRM9 and theobromine was not the secondary metabolite of caffeine degradation. Conclusion, significance and impact of study: Pseudomonas monteilii KRM9 was detected as a new isolate of caffeine-degrading bacteria. This bacterium can be introduced as an agent to degrade caffeine from coffee pulp waste. It is expected that further research can be conducted on the overall mechanism of caffeine degradation by P. monteilii KRM9.
采用超高效液相色谱-串联质谱法快速同步分析人体体液中的精神药物
DOI：
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发表时间：
2009
期刊：
影响因子：
--
作者：
金子理奈;et al.
通讯作者：
et al.