A novel fusion transcription factor drives high cellulase and xylanase production on glucose in Trichoderma reesei
A novel fusion transcription factor drives high cellulase and xylanase production on glucose in Trichoderma reesei
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DOI：
10.1016/j.biortech.2022.128520
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发表时间：
2022-12-27
影响因子：
11.4
通讯作者：
Liu, Weifeng
中科院分区：
文献类型：
--
作者：
Lv, Dongmei;Zhang, Weixin;Liu, Weifeng
为了降低木质纤维素生物精炼中（半）纤维素酶生产的高成本，制定策略以提高经济且易于操作的碳源的酶生产率非常重要。在本研究中，通过将Xyr1的DNA结合域与Tmac1的反式激活域融合，设计了人工转录因子XT。当在里氏木霉 QM9414.xyr1 中过表达时，与 Avicel 上的 QM9414 相比，XT 重组菌株 (OEXT) 大大提高了抑制葡萄糖的（半）纤维素酶产量，pNPCase 和木聚糖酶活性分别增加了 1.7 倍和 8.2 倍。这两种活性甚至比同样过表达 Xyr1 的重组菌株更高（分别高 0.9 倍和 33.8 倍）。 OEXT 中木聚糖酶活性的显着增强是由于分泌蛋白组中各种半纤维素酶的表达升高所致。此外，与等体积负载的 QM9414 酶相比，OEXT 酶混合物将玉米秸秆的糖化效率提高了 60%。
To reduce the high cost of (hemi)cellulase production in lignocellulose biorefining, it is important to develop strategies to enhance enzyme productivity from economic and also readily manipulatable carbon sources. In this study, an artificial transcription factor XT was designed by fusing the DNA binding domain of Xyr1 to the transactivation domain of Tmac1. When overexpressed in Trichoderma reesei QM9414.xyr1, the XT recombinant strain (OEXT) greatly improved (hemi)cellulase production on repressing glucose compared with QM9414 on Avicel with 1.7- and 8.2-fold increases in pNPCase and xylanase activity, respectively. Both activities were even higher (0.9- and 33.8-fold higher, respectively) than the recombinant strain similarly overexpressing Xyr1. The dramatically enhanced xylanase activities in OEXT resulted from the elevated expression of various hemicellulases in the secretome. Moreover, the enzyme cocktail from OEXT improved the saccharification efficiency toward corn stover by 60% compared with enzymes from QM9414 with equal volume loading.