Extracellular replication of Listeria monocytogenes in the murine gall bladder
Extracellular replication of Listeria monocytogenes in the murine gall bladder
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DOI：
10.1126/science.1092712
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发表时间：
2004-02-06
期刊：
影响因子：
56.9
通讯作者：
Contag, CH
中科院分区：
文献类型：
--
作者：
Hardy, J;Francis, KP;Contag, CH
单核细胞增生李斯特菌可引起人类危及生命的全身性疾病。尽管几十年来在李斯特菌病动物模型方面取得了进展，但对感染和定植过程仍有许多未知之处。本文报道单核增生乳杆菌可在小鼠胆囊中复制，并证明其复制存在于细胞外和腔内。采用体内生物发光成像来确定活体动物感染的位置，在患病和无症状的动物中，在数天内显示来自胆囊的强烈信号。这些资料提示单核增生乳杆菌可能在人胆囊中携带。
The bacterium Listeria monocytogenes can cause a life-threatening systemic illness in humans. Despite decades of progress in animal models of listeriosis, much remains unknown about the processes of infection and colonization. Here, we report that L. monocytogenes can replicate in the murine gall bladder and provide evidence that its replication there is extracellular and intraluminal. In vivo bioluminescence imaging was employed to determine the location of the infection over time in live animals, revealing strong signals from the gall bladder over a period of several days, in diseased as well as asymptomatic animals. The data suggest that L. monocytogenes may be carried in the human gall bladder.