Application of the BED capture enzyme immunoassay for HIV incidence estimation among female sex workers in Kaiyuan City, China, 2006-2007
Application of the BED capture enzyme immunoassay for HIV incidence estimation among female sex workers in Kaiyuan City, China, 2006-2007
复制标题
DOI：
10.1016/j.ijid.2009.09.004
复制
发表时间：
2010-07-01
影响因子：
8.4
通讯作者：
Wang, Ning
中科院分区：
文献类型：
--
作者：
Xu, Junjie;Wang, Haibo;Wang, Ning
目的：采用连续横断面调查和免疫球蛋白捕获床酶免疫试验(Bed-CEIA)方法，对开原市暗娼人群进行为期6个月的3次横断面调查，了解暗娼中HIV感染情况。对HIV抗体阳性的样本进行Bed-CEIA检测。结果：在1412名独立参与者中，475名HIV抗体检测为阴性并参加了>；1调查(纵向队列)。与只参与一次的786名HIV阴性的暗娼相比，纵向队列报告了更多的非法药物使用(10.9%比7.4%，p=0.03)，在前3个月注射毒品更频繁(8.8%比5.3%，p=0.02)，尿阿片试验阳性(13.7%比8.9%，p=0.008)。纵向队列中的4名参与者在过去一年中血清转换，总发病率为1.1/100人年(95%可信区间(CI)0.3-2.8)。粗床CEIA发病率为3.4/100人年(95%可信区间2.3-4.4)，调整后的发病率与队列发病率相似：麦克道格尔1.5/100人年(95%可信区间1.0-2.0)；哈格罗夫1.6/100人年(95%可信区间1.1-2.1)。在已知感染天数的暗娼样本中，Bed-CEIA假阳性率为4.4%(10/229)。结论：本研究虽然受到力量的限制，但为中国地区Bed-CEIA的验证提供了补充数据。如果得到其他研究的证实，Bed-CEIA将成为估计艾滋病毒发病率和趋势的有用工具。(C)2010年国际传染病学会。爱思唯尔有限公司出版。保留所有权利。
Objective: To estimate HIV incidence among female sex workers (FSWs) by serial cross-sectional surveys and IgG-capture BED-enzyme immunoassay (BED-CEIA).Methods: We conducted three cross-sectional surveys, 6 months apart, among all consenting FSWs in Kaiyuan City, China. HIV antibody-positive samples were also tested by BED-CEIA.Results: Among 1412 unique participants, 475 tested HIV-negative and attended >1 survey (longitudinal cohort). Compared to 786 HIV-negative FSWs who only participated once, the longitudinal cohort reported more illicit drug use (10.9% vs. 7.4%, p = 0.03), injected drugsmore often in the previous 3 months (8.8% vs. 5.3%, p = 0.02), and had more positive urine opiate tests (13.7% vs. 8.9%, p = 0.008). Four participants in the longitudinal cohort seroconverted over the year, with an overall incidence of 1.1/100 person-years (95% confidence interval (CI) 0.3-2.8). Crude BED-CEIA incidence was 3.4/100 person-years (95% CI 2.3-4.4) with adjusted rates similar to the cohort incidence: McDougal, 1.5/100 person-years (95% CI 1.0-2.0); Hargrove, 1.6/100 person-years (95% CI 1.1-2.1). The BED-CEIA false-positive rate was 4.4% (10/229) among samples from FSWs known to be infected >= 365 days.Conclusions: Although limited by power, this study provides additional data towards validating BED-CEIA in China. If confirmed by other studies, BED-CEIA will be a useful tool to estimate HIV incidence rates and trends. (C) 2010 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.