Sugars and cuticular waxes impact sugarcane aphid (Melanaphis sacchari) colonization on different developmental stages of sorghum
Sugars and cuticular waxes impact sugarcane aphid (Melanaphis sacchari) colonization on different developmental stages of sorghum
复制标题
糖和角质层蜡影响甘蔗蚜虫（Melanaphis sacchari）在高粱不同发育阶段的定植
DOI：
10.1016/j.plantsci.2023.111646
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发表时间：
2023
期刊：
影响因子：
5.2
通讯作者：
Louis, Joe
中科院分区：
文献类型：
--
作者：
Cardona, Juan Betancurt;Grover, Sajjan;Bowman, Michael J.;Busta, Lucas;Kundu, Pritha;Koch, Kyle G.;Sarath, Gautam;Sattler, Scott E.;Louis, Joe
甘蔗蚜虫（Melanaphis sacchari）是高粱的一种毁灭性害虫，在高粱的不同生长阶段定殖。蚜虫在寄主植物上的定居和取食往往受叶片表面特征和糖类的影响。然而，角质层蜡质和糖水平的变化如何影响SCA的建立和高粱不同发育阶段的喂养还没有探索。在这项研究中，两个和六周龄BTx 623植物，高粱的参考线，被用来评估植物蚜虫的相互作用。利用电子穿透图（EPG）技术监测蚜虫的取食行为发现，蚜虫花更多的时间在筛元件阶段相比，两周龄的植物，6周龄的植物。显着差异被发现在所花费的时间达到第一筛元件和途径阶段之间的两个和六个星期的老厂。然而，无选择的蚜虫生物测定显示，SCA的人口数较高，在两个星期的老厂相比，六个星期的老厂。分析了蜡和糖含量丰度的差异，以确定这些植物成分如何影响蚜虫取食和增殖。在分析的表皮蜡化合物中，仅在6周龄的植物中，在蚜虫侵染10天后，α-香树脂醇和异木脂酮增加。SCA饲喂两周和六周大的植物后，海藻糖含量显着增加。此外，SCA喂养抑制蔗糖含量和葡萄糖和果糖的水平增加，在两个星期的老，但不是在六个星期的老厂。总体而言，我们的研究表明，植物年龄是SCA喂养的决定因素，三萜类化合物和有效糖的微妙变化影响SCA建立高粱植物。
Sugarcane aphid (SCA;Melanaphis sacchari) is a devastating pest of sorghum (Sorghum bicolor) that colonizes sorghum plants at different growth stages. Leaf surface characteristics and sugars often influence aphid settling and feeding on host plants. However, how changes in cuticular waxes and sugar levels affect SCA establishment and feeding at different development stages of sorghum have not been explored. In this study, two- and six-week-old BTx623 plants, a reference line of sorghum, was used to evaluate plant-aphid interactions. Monitoring aphid feeding behavior using Electrical Penetration Graph (EPG) technique revealed that aphids spent more time in the sieve element phase of six-week-old plants compared to two-week-old plants. Significant differences were found in the time spent to reach the first sieve element and pathway phases between the two- and six-week-old plants. However, no-choice aphid bioassays displayed that SCA population numbers were higher in two-week-old plants compared to six-week-old plants. Differences in the abundance of wax and sugar contents were analyzed to determine how these plant components influenced aphid feeding and proliferation. Among the cuticular wax compounds analyzed, α-amyrin and isoarborinone increased after 10 days of aphid infestation only in six-week-old plants. Trehalose content was significantly increased by SCA feeding on two- and six-week-old plants. Furthermore, SCA feeding depressed sucrose content and increased levels of glucose and fructose in two-week-old but not in six-week-old plants. Overall, our study indicates that plant age is a determinant for SCA feeding, and subtle changes in triterpenoids and available sugars influence SCA establishment on sorghum plants.