ANALYSIS OF HISTOCOMPATIBILITY REQUIREMENTS FOR PROLIFERATIVE AND HELPER T-CELL ACTIVITY - T-CELL POPULATIONS DEPLETED OF ALLOREACTIVE CELLS BY NEGATIVE SELECTION
ANALYSIS OF HISTOCOMPATIBILITY REQUIREMENTS FOR PROLIFERATIVE AND HELPER T-CELL ACTIVITY - T-CELL POPULATIONS DEPLETED OF ALLOREACTIVE CELLS BY NEGATIVE SELECTION
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DOI：
10.1084/jem.152.5.1311
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发表时间：
1980-01-01
影响因子：
15.3
通讯作者：
DUTTON, RW
中科院分区：
文献类型：
--
作者：
SHIH, WWH;MATZINGER, PC;DUTTON, RW
从用半抗原-载体缀合物免疫的[小鼠]供体制备T细胞群，并通过阴性选择耗尽同种异体反应性细胞。这是通过将细胞注射到H-2不同照射的受体中并在18-40小时后从胸导管中回收来实现的。这些人口的增殖和辅助活动的遗传要求进行了测定。确定了对贴壁Thy-1阴性细胞上呈递的抗原的增殖反应;证明了对同源抗原呈递细胞（APC）的要求。检测相同的T细胞对半抗原致敏的B细胞提供帮助的能力。需要同基因的APC和B细胞和T细胞对同一分子上的半抗原和载体决定簇的连接识别。不要求B细胞与T细胞是H-2相容的。对连锁识别的要求被视为同种异体组合中的反应不是阳性同种异体效应的结果的证据。在阳性选择细胞中发现了精确可比的限制。
T cell populations were prepared from [mouse] donors immunized with hapten-carrier conjugates and were depleted of alloreactive cells by negative selection. This was accomplished by injection of the cells into H-2-disparate irradiated recipients and recovery from the thoracic duct after 18-40 h. The genetic requirements for the proliferative and helper activity of these populations was determined. The proliferative response to antigen presented on adherent, Thy-1-negative cells was determined; a requirement for syngeneic antigen-presenting cells (APC) was demonstrated. The same T cells were assayed for their ability to give help to hapten primed B cells. There was a requirement for syngeneic APC and for linked recognition of hapten and carrier determinants on the same molecule by the B cell and T cell. There was no requirement for the B cell to be H-2 compatible with the T cell. The requirement for linked recognition was taken as evidence that the responses in allogeneic combinations were not a result of positive allogeneic effects. Precisely comparable restrictions were found with positively selected cells.