PRODUCTION AND CHARACTERIZATION OF MOUSE MONOCLONAL-ANTIBODIES DIRECTED AGAINST CANINE IGE AND IGG
PRODUCTION AND CHARACTERIZATION OF MOUSE MONOCLONAL-ANTIBODIES DIRECTED AGAINST CANINE IGE AND IGG
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DOI：
10.1016/0165-2427(93)90193-8
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发表时间：
1993-08-01
影响因子：
1.8
通讯作者：
SCHULTZ, KT
中科院分区：
文献类型：
--
作者：
DEBOER, DJ;EWING, KM;SCHULTZ, KT
在犬中很少研究速发型超敏反应，部分原因是犬IgE特异性抗血清的可用性有限。制备了一系列鼠杂交瘤细胞系，其产生对犬免疫球蛋白E（IgE）和IgG具有特异性的单克隆抗体（MAb）。检测了MAb在犬皮肤中诱导逆转皮肤过敏反应、中和特应性犬血清的Prausnitz-Kustner反应性、在免疫亲和色谱中用作配体以及在几种ELISA系统中与IgE和其他IG亚类结合的能力。发现产生的一些单克隆抗体对犬IgE具有特异性。其他单克隆抗体承认共同或相似的决定因素，IgE和IgG，或IgG和IgM，虽然有明显不同的亲和力。犬IgE的热或酸处理消除了大部分（但不是全部）与抗IgE单克隆抗体的反应性。这些单克隆抗体将有助于进一步研究IgE介导的现象，在狗。
Immediate-type hypersensitivity reactions have been studied infrequently in dogs, in part because of limited availability of antisera specific for canine IgE. A series of murine hybridoma cell lines were prepared, that produced monoclonal antibodies (MAb) with specificity for canine immunoglobulin E (IgE) and IgG. The MAb were tested for their ability to induce a reverse cutaneous anaphylaxis reaction in dog skin, to neutralize the Prausnitz-Kustner reactivity of atopic dog serum, to serve as a ligand in immunoaffinity chromatography, and to bind to IgE and other Ig subclasses in several ELISA systems. Some of the MAb produced were found to be specific for canine IgE. Other MAb recognized common or similar determinants on IgE and IgG, or on IgG and IgM, though with apparently differing affinities. Heat or acid treatment of canine IgE abolished most, but not all, of the reactivity with the anti-IgE MAb. These MAb will be useful for further study of IgE-mediated phenomena in the dog.