The Oligosaccharyltransferase AgIB Supports Surface-Associated Growth and Iron Oxidation in Methanococcus maripaludis
The Oligosaccharyltransferase AgIB Supports Surface-Associated Growth and Iron Oxidation in Methanococcus maripaludis
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DOI：
10.1128/aem.00995-21
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发表时间：
2021-09-01
影响因子：
4.4
通讯作者：
Costa，Kyle C.
中科院分区：
文献类型：
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作者：
Holten，Matthew P.;Fonseca，Dallas R.;Costa，Kyle C.
大多数微生物以表面附着的群落形式生长，称为生物膜。然而，产甲烷古菌生长附着在表面的机制仍然研究不足。在这里，我们表明，寡糖基转移酶AglB是必不可少的甲烷球菌菌株JJ在玻璃或金属表面上的生长。AglB糖基化几种细胞结构，如皮利、古细菌和细胞表面层（S层）。我们表明，S-层的菌株JJ，但不是菌株S2，是一种糖蛋白，只有菌株JJ是能够在表面上生长，和删除的aglB阻断S-层糖基化和废除表面相关的增长。缺乏IV型菌毛结构成分的菌株JJ突变体在任何测试条件下都没有生长缺陷，而缺乏前鞭毛蛋白肽酶（ΔflaK）的突变体只有在甲酸盐作为唯一电子供体时才有表面生长缺陷。最后，对于能够Fe 0氧化的菌株，我们表明，删除的aglB降低厌氧Fe 0氧化的速率，大概是由于减少与Fe 0表面的生物量。总之，这些数据提供了一个初始表征的产甲烷古菌的成员中的表面相关的增长。IMPORTANCEMethanogenic archaea负责生产地球上的大部分甲烷和催化缺氧环境中的有机物降解的终端反应。产甲烷菌通常作为与表面或伙伴生物体相关的生物膜生长;然而，表面相关生长的分子细节仍然没有表征。我们已经发现证据表明，糖基化的细胞表面层是必不可少的生长ofM。maripaludison表面，可以提高厌氧铁腐蚀率。这些结果提供了深入了解表面相关的产甲烷生物的生理学，并强调了厌氧铁腐蚀的表面协会的重要性。
Most microbial organisms grow as surface-attached communities known as biofilms. However, the mechanisms whereby methanogenic archaea grow attached to surfaces have remained understudied. Here, we show that the oligosaccharyltransferase AglB is essential for growth of Methanococcus maripaludis strain JJ on glass or metal surfaces. AglB glycosylates several cellular structures, such as pili, archaella, and the cell surface layer (S-layer). We show that the S-layer of strain JJ, but not strain S2, is a glycoprotein, that only strain JJ was capable of growth on surfaces, and that deletion ofaglBblocked S-layer glycosylation and abolished surface-associated growth. A strain JJ mutant lacking structural components of the type IV-like pilus did not have a growth defect under any conditions tested, while a mutant lacking the preflagellin peptidase (ΔflaK) was defective for surface growth only when formate was provided as the sole electron donor. Finally, for strains that are capable of Fe0oxidation, we show that deletion ofaglBdecreases the rate of anaerobic Fe0oxidation, presumably due to decreased association of biomass with the Fe0surface. Together, these data provide an initial characterization of surface-associated growth in a member of the methanogenic archaea.IMPORTANCEMethanogenic archaea are responsible for producing the majority of methane on Earth and catalyze the terminal reactions in the degradation of organic matter in anoxic environments. Methanogens often grow as biofilms associated with surfaces or partner organisms; however, the molecular details of surface-associated growth remain uncharacterized. We have found evidence that glycosylation of the cell surface layer is essential for growth ofM. maripaludison surfaces and can enhance rates of anaerobic iron corrosion. These results provide insight into the physiology of surface-associated methanogenic organisms and highlight the importance of surface association for anaerobic iron corrosion.