Integrins αvβ3 and α5β1 mediate attachment of Lyme disease spirochetes to human cells
Integrins αvβ3 and α5β1 mediate attachment of Lyme disease spirochetes to human cells
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DOI：
10.1128/iai.66.5.1946-1952.1998
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发表时间：
1998-05-01
影响因子：
3.1
通讯作者：
Leong, JM
中科院分区：
文献类型：
--
作者：
Coburn, J;Magoun, L;Leong, JM
莱姆病病原体伯氏疏螺旋体（广义）能够在人类和动物宿主中引起慢性、多系统感染。螺旋体与宿主细胞的附着对于不同组织的定殖可能是重要的。血小板特异性整合素α（II B）β（3）先前被鉴定为所有三种莱姆病螺旋体（B. burgdorferi sensu stricto，B. garinii和B. afzelii）。这里我们展示了B。Burgdorferi还识别广泛表达的整合素α（V）β（3）和α（5）β（1），分别称为玻连蛋白和纤连蛋白受体。测试每种莱姆病螺旋体的三个代表与纯化的α（v）β（3）和α（5）β（1）结合的能力。所有测试的菌株都与至少一种整联蛋白结合。与一种整联蛋白的结合并不总是预测与其他整联蛋白的结合，并且鉴定了几种不同的整联蛋白偏好谱。传染性B附着。通过RGD肽和适当的受体特异性抗体抑制伯氏菌菌株N40与纯化的α（v）β（3）和α（5）β（1）的结合。通过使用表达该受体但不表达α（III）β（3）的转染细胞系，也显示了与α（V）β（3）的结合。附件B。BurgdorferiN40对人红白血病细胞和人隐静脉内皮细胞的作用由α（5）β（1）和α（v）β（3）介导。我们的研究结果表明，多种整合素介导的莱姆病螺旋体附着到宿主细胞。
Borrelia burgdorferi (sensu lato), the agent of Lyme disease, is able to cause chronic, multisystemic infections in human and animal hosts. Attachment of the spirochete to host cells is likely to be important for the colonization of diverse tissues. The platelet-specific integrin alpha(IIb)beta(3) was previously identified as a receptor for all three species of Lyme disease spirochetes (B. burgdorferi sensu stricto, B. garinii, and B. afzelii). Here we show that B. burgdorferi also recognizes the widely expressed integrins alpha(v) beta(3) and alpha(5) beta(1), known as the vitronectin and fibronectin receptors, respectively. Three representatives of each species of Lyme disease spirochete were tested for the ability to bind to purified alpha(v) beta(3) and alpha(5) beta(1). All of the strains tested bound to at least one integrin. Binding to one integrin was not always predictive of binding to other integrins, and several different integrin preference profiles were identified. Attachment of the infectious B. burgdorferi strain N40 to purified alpha(v) beta(3) and alpha(5) beta(1), was inhibited by RGD peptides and the appropriate receptor-specific antibodies. Binding to alpha(v) beta(3) was also shown by using a transfected cell line that expresses this receptor but not alpha(III)beta(3). Attachment of B. burgdorferi N40 to human erythroleukemia cells and to human saphenous vein endothelial cells was mediated by both alpha(5) beta(1) and alpha(v) beta(3). Our results show that multiple integrins mediate attachment of Lyme disease spirochetes to host cells.