Separations of Carbohydrates with Noncovalent Shift Reagents by Frequency-Modulated Ion Mobility-Orbitrap Mass Spectrometry
Separations of Carbohydrates with Noncovalent Shift Reagents by Frequency-Modulated Ion Mobility-Orbitrap Mass Spectrometry
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DOI：
10.1021/jasms.1c00184
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发表时间：
2021-08-05
影响因子：
3.2
通讯作者：
Fernandez, Facundo M.
中科院分区：
文献类型：
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作者：
McKenna, Kristin R.;Clowers, Brian H.;Fernandez, Facundo M.
由于碳水化合物在现存生命中的许多重要作用以及在化学进化和生命起源中的潜在作用，人们越来越关注碳水化合物的结构异质性。本工作建立了多路漂移管离子迁移率-Orbitrap质谱仪分析非共价移位试剂修饰的双糖混合物。由于大气压漂移管离子迁移率池与Orbitrap质谱仪的传统耦合存在占空比低(
An increased focus on characterizing the structural heterogeneity of carbohydrates has been driven by their many significant roles in extant life and potential roles in chemical evolution and the origin of life. In this work, multiplexed drift tube ion mobility-Orbitrap mass spectrometry methods were developed to analyze mixtures of disaccharides modified with noncovalent shift reagents. Since traditional coupling of atmospheric pressure drift tube ion mobility cells with Orbitrap mass analyzers suffers from low duty cycles (