CHARACTERIZATION AND EXPRESSION ANALYSIS OF THE MURINE RCK GENE - A PROTEIN-KINASE WITH A POTENTIAL FUNCTION IN SENSORY CELLS
CHARACTERIZATION AND EXPRESSION ANALYSIS OF THE MURINE RCK GENE - A PROTEIN-KINASE WITH A POTENTIAL FUNCTION IN SENSORY CELLS
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DOI：
10.1111/j.1432-0436.1993.tb00651.x
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发表时间：
1993-06-01
期刊：
影响因子：
2.9
通讯作者：
BIRCHMEIER, C
中科院分区：
文献类型：
--
作者：
BLADT, F;BIRCHMEIER, C
我们的特点是鼠蛋白激酶基因，rck，这是确定的交叉杂交与序列的v-ros酪氨酸激酶基因的条件下，减少严格性。cDNA分析表明，rck编码一个推定的蛋白激酶相关的cdc 2亚类的基因家族，该基因是相同的，以前在大鼠中确定的mak。通过原位杂交和RNA酶保护的组合在小鼠中进行的广泛的表达分析揭示了一种新的和限制性的表达模式：rck转录被发现在两种细胞类型中参与感觉传导，光感受器和嗅觉受体以及在呼吸道和脉络丛的上皮细胞。在减数分裂前和减数分裂后的雄性生殖细胞中也发现了特异性转录物。因此，我们认为rck参与了一组独特的细胞中重要的信号传导途径，尤其是在感觉信号转导细胞中。
We have characterized a murine protein kinase gene, rck, which was identified by crosshybridization with sequences from the v-ros tyrosine kinase gene under conditions of reduced stringency. cDNA analysis indicates that rck encodes a putative protein kinase related to the cdc2 subclass of the gene family and that the gene is identical to mak identified previously in the rat. An extensive expression analysis in the mouse performed by a combination of in situ hybridization and RNase protection revealed a novel and restricted pattern of expression: rck transcripts are found in two cell types involved in sensory transduction, photoreceptors and olfactory receptors as well as in epithelia of the respiratory tract and choroid plexus. Specific transcripts are also found in pre- and postmeiotic male germ cells. We suggest therefore that rck participates in signalling pathways important in a distinct set of cells, remarkably among them cells involved in sensory signal transduction.