Pseudomonas aeruginosa cytochrome c551 denaturation by five systematic urea derivatives that differ in the alkyl chain length.
Pseudomonas aeruginosa cytochrome c551 denaturation by five systematic urea derivatives that differ in the alkyl chain length.
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铜绿假单胞菌细胞色素 c551 被五种烷基链长度不同的系统脲衍生物变性。
DOI：
10.1080/09168451.2017.1303361
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Y
中科院分区：
文献类型：
--
作者：
78.Kobayashi;S.;Fujii;S.;Koga;A.;Wakai;S.,Matubayasi;N.;Sambongi;Y
用5种不同烷基链长的脲衍生物，即脲、N-甲基脲（MU）、N-乙基脲（EU）、N-丙基脲（PU）和N-丁基脲（BU），对铜绿假单胞菌胞色素551（PAC 551）进行可逆变性研究。PAC 551变性所需的BU浓度最低，PU、EU、MU和尿素的浓度逐渐升高。BU引起的PAC 551变性后的可及表面积差异最大，PU、EU、MU和尿素引起的可及表面积差异逐渐减小。这些发现表明具有较长烷基链的脲衍生物是较强的变性剂。在本研究中，多达五个系统的脲衍生物可以应用于一个单一的蛋白质，PAC 551的可逆变性的第一次，和烷基链的长度对蛋白质变性的影响进行了系统的验证，通过热力学参数。
Reversible denaturation ofPseudomonas aeruginosacytochromec551(PAc551) could be followed using five systematic urea derivatives that differ in the alkyl chain length, i.e. urea,N-methylurea (MU),N-ethylurea (EU),N-propylurea (PU), andN-butylurea (BU). The BU concentration was the lowest required for the PAc551denaturation, those of PU, EU, MU, and urea being gradually higher. Furthermore, the accessible surface area difference upon PAc551denaturation caused by BU was found to be the highest, those by PU, EU, MU, and urea being gradually lower. These findings indicate that urea derivatives with longer alkyl chains are stronger denaturants. In this study, as many as five systematic urea derivatives could be applied for the reversible denaturation of a single protein, PAc551, for the first time, and the effects of the alkyl chain length on protein denaturation were systematically verified by means of thermodynamic parameters.