Comparison between genetic portraits of osteoblasts derived from primary cultures and osteoblasts obtained from human pulpar stem cells
Comparison between genetic portraits of osteoblasts derived from primary cultures and osteoblasts obtained from human pulpar stem cells
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DOI：
10.1097/scs.0b013e31816aabc8
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发表时间：
2008-05-01
影响因子：
0.9
通讯作者：
Pezzetti, Furio
中科院分区：
文献类型：
--
作者：
Carinci, Francesco;Papaccio, Gianpaolo;Pezzetti, Furio
获取骨进行自体移植是颅面和口腔外科医生每天都会遇到的问题。来源于人牙髓的干细胞能够在成骨细胞中分化，是体外产生自体骨的潜在来源。然而，由于干细胞具有自我更新和在几种细胞亚型(即多潜能分化)中的承诺的特点，其潜在的不受控制的增殖可能引起一些关注。为了筛选人牙髓干细胞(ODHPSCs)来源的成骨细胞的行为，我们使用基因芯片技术来鉴定ODHPSC中与正常成骨细胞(NOS)不同的调控基因。从人牙髓中获得人牙髓干细胞来源的成骨细胞，用流式细胞仪进行细胞筛选。细胞型为c-kit(+)/CD34(+)/Stro-1(+)/CD45(-)。通过包含19,200个基因的DNA芯片，我们在ODHPSC中发现了一些与NO相比表达显著上调和下调的基因。这些差异表达的基因具有不同的功能活性：(A)细胞分化，(B)发育成熟，(C)细胞黏附，(D)细胞骨架元素的产生。因此，尽管先前考虑的组织学参数显示出正常的表型，但NO和ODHPSC之间存在一些分子差异。
Harvesting bone for autologous grafting is a daily problem encountered by craniofacial and oral surgeons. Stem cells derived from human dental pulp are able to differentiate in osteoblasts and are a potential source of autologous bone produced in vitro. However, as stem cells are characterized by self-renewing and commitment in several cellular subtypes (ie, pluripotential differentiation), some concerns may arise as regards their potential uncontrolled proliferation.To screen the behavior of osteoblasts derived from human pulpar stem cells (ODHPSCs), we used microarray techniques to identify genes that are differently regulated in ODHPSC in comparison to normal osteoblasts (NOs). Osteoblasts derived from human pulpar stem cells were obtained from human dental pulp, and cells were selected using a cytometer. The cell profile was c-kit(+)/CD34(+)/ STRO-1(+)/CD45(-). These cells were capable of differentiation of osteoblasts in vitro.By using DNA microarrays containing 19,200 genes, we identified in ODHPSC some genes whose expression was significantly up- and down-regulated compared to NO. The differentially expressed genes have different functional activities: (a) cell differentiation, (b) developmental maturation, (c) cell adhesion, and (d) production of cytoskeleton elements.Thus, some molecular differences exist between NO and ODHPSC, although the previously considered histologic parameters show a normal phenotype.