Fabrication of high-capacity biomolecular carriers from dispersible single-walled carbon nanotube-polymer composites.
Fabrication of high-capacity biomolecular carriers from dispersible single-walled carbon nanotube-polymer composites.
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DOI：
10.1021/la9015349
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Pu Zhang;D. Henthorn
中科院分区：
文献类型：
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作者：
Pu Zhang;D. Henthorn
碳纳米管最有趣的应用之一是作为生物分析设备的支撑材料。在这项工作中，我们成功地使用了紫外光引发的“接枝”聚合方法来制造聚合物功能化的碳纳米管（PFCNT）与各种功能的侧链，包括聚（乙二醇）链，以提高蛋白质缀合物的网站的稳定性和悬垂的环氧基团。一种模型酶，碱性磷酸酶，被用来研究生物分子负载效率以及酶活性的保留。制造具有不同比例的两种单体的样品以优化它们在水性环境中的使用，并确定最佳组合物。该方法允许在保持高酶活性的同时提高酶装载量。利用STEM和AFM对功能化前后碳纳米管的形貌进行了表征。此外，通过FT-IR、TGA和XPS对所得PFCNTs进行了分析。
One of the most interesting applications for carbon nanotubes is as a support material for bioanalytical devices. In this work, we successfully used an ultraviolet light initiated "graft from" polymerization method to fabricate polymer functionalized carbon nanotubes (PFCNTs) with pendant chains of various functionalities, including poly(ethylene glycol) chains to boost dispersibility and pendant epoxy groups for protein conjugate sites. A model enzyme, alkaline phosphatase, was used to study biomolecule loading efficiency as well as the retention of enzyme activity. Samples with various ratios of the two monomers were fabricated to optimize their use in aqueous environments, and an optimal composition was determined. This method allows the enhancement of enzyme loading amount while retaining high enzyme activity. The morphology of the carbon nanotubes were characterized by STEM and AFM before and after functionalization. In addition, the resulting PFCNTs were analyzed by FT-IR, TGA, and XPS.