Fabrication of biomimetic resorption lacunae-like structure on titanium surface and its osteoblast responses
Fabrication of biomimetic resorption lacunae-like structure on titanium surface and its osteoblast responses
复制标题
钛表面仿生吸收凹坑状结构的制备及其成骨细胞反应
DOI：
10.1016/j.apsusc.2017.11.282
复制
发表时间：
2018-04-01
影响因子：
6.7
通讯作者：
Tian, Weidong
中科院分区：
文献类型：
--
作者：
Huo, Fangjun;Guo, Weihua;Tian, Weidong
仿生特定表面结构可以改善特定细胞的生物学行为，最终实现组织整合。钛表面与骨吸收腔隙（RL）相似的结构是改善成骨细胞反应和骨整合的一种很有前途的方法。喷砂酸蚀（SLA）表面是最常用的种植体表面，其微观结构与RL表面具有相似的尺寸，但在形状和轮廓上存在较大差异。在这项工作中，通过在新型HCOONa/CH3COONa电解液中阳极氧化钛衬底，制备了具有高度相似尺寸，形状和轮廓的RL-like火山口结构。与SLA相比，它在形状和轮廓上更接近RL结构。此外，通过随后的碱热处理，获得了覆盖整个表面的纳米级结构，进一步模拟了RL内部的凹痕特征。制备的表面由结晶二氧化钛组成，具有超亲水性和良好的稳定性。体外评价结果显示，与SLA相比，该表面能显著改善MG63细胞的粘附、增殖和分化。这种新方法可能是一种很有前途的仿生修复钛种植体以促进骨整合的候选方法。(C) 2017 Elsevier B.V.版权所有
Biomimetic specific surface structure could improve biological behaviors of specific cells and eventual tissue integration. Featuring titanium surface with structures resembling bone resorption lacunae (RL) can be a promising approach to improve the osteoblast responses and osseointegration of implants. As a most common used dental implant surface, sandblasting and acid etching (SLA) surface has micro-sized structures with dimensions similar to RL, but great differences exist when it comes to shape and contour. In this work, by anodizing titanium substrate in a novel HCOONa/CH3COONa electrolyte, RL-like crater structures were fabricated with highly similar size, shape and contour. Compared with SLA, it was much more similar to RL structure in shape and contour. Furthermore, through subsequent alkali-heat treatment, nano-sized structures that overlaid the whole surface were obtained, which further mimic undercuts features inside the RL. The as-prepared surface was consisted of crystalline titania and exhibited super-hydrophilicity with good stability. In vitro evaluation results showed that the surface could significantly improve adhesion, proliferation and differentiation of MG63 cells in comparison with SLA. This new method may be a promising candidate for biomimetic modification of titanium implant to promote osseointegration. (C) 2017 Elsevier B.V. All rights reserved.