Selective down-regulation of high-affinity IgE receptor (FcεRI) α-chain messenger RNA among transcriptome in cord blood-derived versus adult peripheral blood-derived cultured human mast cells
Selective down-regulation of high-affinity IgE receptor (FcεRI) α-chain messenger RNA among transcriptome in cord blood-derived versus adult peripheral blood-derived cultured human mast cells
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DOI：
10.1182/blood.v97.4.1016
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发表时间：
2001-02-15
期刊：
影响因子：
20.3
通讯作者：
Saito, H
中科院分区：
文献类型：
--
作者：
Iida, M;Matsumoto, K;Saito, H
大量的人肥大细胞（MCs）是从脐带血（CB）和成人外周血（PB）中产生的。单个CB祖细胞产生15436个MCs，而单个PB祖细胞平均产生807个MCs。然而，就高亲和力IgE受体（Fc epsilon RI）介导的反应而言，pb衍生的MCs远比cb衍生的MCs活跃。100万个致敏的pb来源的MCs释放3.6杯组胺，215 pg IL-5和14 ng粒细胞巨噬集落刺激因子（GMCSF），而10(6)个致敏的cb来源的MCs仅释放0.8杯组胺，31 pg IL-5和0.58 ng GM-CSF。然而，离子载体a23187从2种MC类型中释放出相似水平的组胺。pr来源的MCs高表达表面FceRI α链，而cb来源的MCs在缺乏IgE的情况下几乎缺乏FceRI α链，pr来源的MCs表达的Fc epsilon RI a链信使RNA （mRNA）水平约为cb来源的MCs的5倍，但受体的β链和γ链mRNA的表达水平相同。在高密度寡核苷酸探针阵列系统检测的约5600种全长人类基因中，Fc epsilon RI α在pb来源的MC中排名第5位，其他4个增加的转录本与MC功能无关。这些结果表明，通过选择性抑制Fc epsilon RI α转录，ige介导的反应可能在婴儿早期受到限制，这可能在祖细胞阶段发生，至少部分是细胞因子不敏感的。(C) 2001年由美国血液学会出版。
Substantial numbers of human mast cells (MCs) were generated from umbilical cord blood (CB) and from adult peripheral blood (PB). A single CB progenitor produced 15 436 MCs, whereas a single PB progenitor produced 807 MCs on average. However, PB-derived MCs were far more active than CB-derived MCs in terms of high-affinity IgE receptor (Fc epsilon RI)-mediated reactions. One million sensitized PB-derived MCs released 3.6 mug histamine, 215 pg IL-5, and 14 ng granulocyte macrophage-colony-stimulating factor (GMCSF), whereas 10(6) sensitized CB-derived MCs released only 0.8 mug histamine, 31 pg IL-5, and 0.58 ng GM-CSF on anti-IgE challenge. However, ionophore A23 187 released similar levels of histamine from the 2 MC types. PR-derived MCs highly expressed surface FceRI alpha chain, and CB-derived MCs almost lacked it in the absence of IgE, PR-derived MCs expressed approximately 5 times higher levels of messenger RNA (mRNA) for Fc epsilon RI a chain than CB-derived MCs, but mRNAs for beta and gamma chains of the receptors were equally expressed. Among the approximately 5600 kinds of full-length human genes examined by using the high-density oligonucleotide probe-array system, Fc epsilon RI alpha was ranked the fifth most increased transcript in PB-derived MCs, The 4 other increased transcripts were unrelated to MC function. These results suggest that IgE-mediated reactions may be restricted during early infancy through the selective inhibition of Fc epsilon RI alpha transcription, which is probably committed at progenitor stages and is, at least in part, cytokine-insensitive. (C) 2001 by The American Society of Hematology.