Verification of differentially expressed genes in relation to hydrostatic pressure in the brain of two wrasse species with high‐tide preference in spawning
Verification of differentially expressed genes in relation to hydrostatic pressure in the brain of two wrasse species with high‐tide preference in spawning
复制标题
验证与产卵高潮偏好的两种濑鱼大脑中静水压相关的差异表达基因
DOI：
10.1111/jfb.14458
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发表时间：
2020
影响因子：
2
通讯作者：
Akihiro Takemura
中科院分区：
文献类型：
--
作者：
Shingo Udagawa;Sung-Pyo Hur;Jun-Hwan Byun;Hiroki Takekata;Yuki Takeuchi;Akihiro Takemura
生活在浅水中的鱼类会受到潮汐信号的周期性变化，包括静水压力（HP）。本研究的目的是验证三斑水濑鱼Halichoeres trimaculatus（热带物种）和honbera wrasseHalichoeres tenuispinis（温带物种）大脑中的差异表达基因（DEG），这两种鱼都暴露于30 kPa（现场可能的高潮刺激）或1 kPa（低潮）的HP 3或6 h。Ade novoassembly从threespot wrasse的脑中产生了174，710个重叠群（63，530个重叠群被注释）。RNA测序后，定量PCR证实DEG上调[AT非典型钙粘蛋白2（FAT 2）]和下调[神经元富亮氨酸重复序列蛋白3（LRRN 3）、双特异性酪氨酸磷酸化调节激酶1（DYRK）、丝裂原活化蛋白激酶激酶1（MAP 2K 1）和磷酸肌醇3激酶（PI 3 K）]。HP对这些DEGs（MAP 2K 1除外）转录的影响在6 h内消失，表明HP是一种短暂的刺激，发生在潮汐周期的开始。原位杂交结果显示，MPA 2K 1在端脑、间脑和垂体中有较强的信号，而PI 3 K则在端脑、间脑和延髓中有较强的信号。这一结果表明，这些激酶参与感觉功能（端脑），躯体和内脏功能（延髓）和神经内分泌系统（间脑和垂体），所有这些都与HP刺激的变化。HP暴露后，honbera wrasse垂体中c-fos的转录增加，表明外部刺激直接或间接激活下丘脑-垂体-性腺轴的激素合成。结论是HP改变了与中枢神经系统神经发育和功能相关的基因表达，并在濑鱼的潮汐相关生殖和摄食中发挥作用。
Fish that inhabit shallow water are exposed to periodic changes in tidal cues, including hydrostatic pressure (HP). The present study aimed at verifying differentially expressed genes (DEGs) in the brain of the threespot wrasseHalichoeres trimaculatus(tropical species) and the honbera wrasseHalichoeres tenuispinis(temperate species), both of which were exposed to HP at 30 kPa (possible high‐tide stimuli in the field) or 1 kPa (low tide) for 3 or 6 h. Ade novoassembly yielded 174,710 contigs (63,530 contigs were annotated) from the brain of threespot wrasse. Following RNA sequencing, quantitative PCR confirmed DEGs that were upregulated [AT atypical cadherin 2 (FAT2)] and downregulated [neuronal leucine‐rich repeat protein 3 (LRRN3), dual specificity tyrosine phosphorylation‐regulated kinase 1 (DYRK), mitogen‐activated protein kinase kinase 1 (MAP2K1) and phosphoinositide 3 kinase (PI3K)]. The effect of HP on the transcription of these DEGs (except forMAP2K1) disappeared within 6 h, suggesting that HP is a transitory stimulus occurring at the beginning of the tidal cycle. Similar DEG transcription was observed in the brain of honbera wrasse maintained under HP for 6 h.In situhybridization of the brain of the threespot wrasse revealed that strong signals ofMPA2K1were seen in the telencephalon, diencephalon and pituitary, whereas those ofPI3Kwere seen in the telencephalon, diencephalon and medulla oblongata. This result suggests that these kinases are involved in sensory function (telencephalon), somatic and visceral function (medullar oblongata) and the neuroendocrine system (diencephalon and pituitary), all of which were related to changes in HP stimuli. Following HP exposure, the transcription ofc‐fosincreased in the pituitary of honbera wrasse, suggesting that external stimuli directly or indirectly activate hormone synthesis at the hypothalamic–pituitary–gonadal axis. It is concluded that HP alters gene expression in relation to neural development and function in the central nervous system and plays a role in exerting tidal‐related reproduction and feeding in wrasses.