A QUANTITATIVE CYTOCHEMICAL METHOD FOR ESTIMATING ALKALINE PHOSPHATASE ACTIVITY
A QUANTITATIVE CYTOCHEMICAL METHOD FOR ESTIMATING ALKALINE PHOSPHATASE ACTIVITY
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DOI：
10.1098/rspb.1955.0067
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发表时间：
1955-01-01
期刊：
影响因子：
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通讯作者：
DAVIES, HG
中科院分区：
文献类型：
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作者：
BARTER, R;DANIELLI, JF;DAVIES, HG
通过使用干涉显微镜，可以定量测量由酶活性产生的磷酸钙的沉积速率。这提供了单个细胞位点酶活性的定量测量。通过这种方法，研究了底物浓度、pH、温度和钙离子浓度的变化对大鼠肾小管刷状缘区域和十二指肠上皮酶活性的影响。还对细胞核进行了一些观察。在标准孵育条件下（Danielli 1953），肾脏切片中释放的至少 97⋅5% 的磷酸盐离子会沉淀，并且一旦沉淀，磷酸钙就不会再溶解。在标准孵育条件下，未检测到肾脏和十二指肠刷状缘的酶活性损失。
By the use of interference microscopy the rate of deposition of calcium phosphate, resulting from enzyme activity, can be measured quantitatively. This provides a quantitative measure of enzyme activity at individual cellular sites. By this method, studies have been made of the effect of variation in substrate concentration, pH, temperature and calcium-ion concentration on the enzymatic activity of the brush border regions of rat-kidney tubules and of duodenal epithelium. Some observations of cell nuclei have also been made. Under standard conditions of incubation (Danielli 1953) at least 97⋅5% of the phosphate ions liberated in a kidney section are precipitated, and once precipitated the calcium phosphate does not redissolve. Under standard conditions of incubation no loss of enzyme activity from brush borders of kidney and duodenum could be detected.