Effect of interferon on virus production from isolated single cells.
Effect of interferon on virus production from isolated single cells.
复制标题
干扰素对分离的单细胞病毒产生的影响。
DOI：
--
复制
发表时间：
1973
期刊：
影响因子：
--
通讯作者：
Simon Eh
中科院分区：
文献类型：
--
作者：
Fleischmann Wr;Simon Eh
总结
微滴用于分离经干扰素处理的感染有孟戈病毒的小鼠L细胞。测定了三个参数，（1）病毒产量的时间，（2）产量/细胞，和（3）产量细胞的百分比。超过97%的对照单细胞产生平均167 p.f.u./大多数细胞在感染后11至14小时产生。用干扰素处理的细胞比对照组产生晚（如果有的话），并且所有细胞都可以在一定程度上受到保护。在治疗和控制细胞，病毒被释放超过一段时间约15分钟。增加挑战病毒的感染的多重性降低了给定的干扰素剂量的有效性，而增加干扰素的浓度增加了潜伏期的长度，减少了产量的大小，并减少了生产细胞的数量。基于这些观察，提出（1）干扰素保护的直接效果是延迟病毒合成中的关键步骤，（2）产量大小和产量数量的减少是由于细胞产生病毒的固有能力的逐渐丧失，和（3）延迟是干扰素或由其诱导的分子的结果，作为完成病毒合成所需的某些细胞或病毒产物的竞争性抑制剂。
Summary
Microdrops were used to isolate interferon treated mouse L cells infected with mengovirus. Three parameters were measured, (1) the time of virus yield, (2) the yield/cell, and (3) the percentage of yielding cells. More than 97% of the control single cells yielded an average of 167 p.f.u./cell with most of them yielding between 11 and 14 h post-infection. Cells treated with interferon yielded later than controls (if at all), and all could be protected to some degree. In both treated and control cells, virus was released over a period of about 15 min. Increasing the multiplicity of infection of challenge virus reduced the effectiveness of a given interferon dose, while increasing the concentration of interferon increased the length of the latent period, decreased the size of the yield, and decreased the number of yielding cells. On the basis of these observations it is proposed that (1) the direct effect of interferon protection is to delay a critical step in virus synthesis, (2) the reduction in yield size and in the number of yielders is due to a progressive loss of the inherent ability of the cell to produce virus, and (3) the delay is the result of interferon, or a molecule induced by it, acting as a competitive inhibitor of some cellular or virus product, needed for the completion of virus synthesis.