Contribution of physicochemical characteristics of nano-oxides to cytotoxicity
Contribution of physicochemical characteristics of nano-oxides to cytotoxicity
复制标题
纳米氧化物的理化特性对细胞毒性的贡献
DOI：
10.1016/j.biomaterials.2010.06.022
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发表时间：
2010-11-01
期刊：
影响因子：
14
通讯作者：
Hanagata, Nobutaka
中科院分区：
文献类型：
--
作者：
Xu, Mingsheng;Fujita, Daisuke;Hanagata, Nobutaka
为了确定纳米氧化物的关键物理化学性质的细胞毒性，我们调查的大小，形状，形态和电子性质的10种类型的绝缘体（SiO2，CeO2和Al2O3）和半导体（ZnO和CuO）纳米氧化物的细胞毒性使用NIH 3T3和A549细胞系作为模型的贡献。我们发现，Al2O3的形状（纳米粒子与纳米晶须）和SiO2的形态（多孔与无孔纳米粒子）没有明显的影响所观察到的细胞毒性，和纳米氧化物的大小不能被视为细胞毒性的指标。相比之下，我们发现，暴露于半导体纳米氧化物的细胞活力远低于暴露于绝缘体纳米氧化物。此外，Al掺杂的ZnO纳米颗粒（NP）比未掺杂的ZnO NP毒性更大，而Al掺杂的CuO NP比未掺杂的CuO NP毒性更小，但比Al2O3 NP毒性更大。相应地，纳米氧化物的价带X射线光电子能谱表明，Al掺杂的ZnO NP（Al掺杂的CuO NP）的态密度高于（低于）未掺杂的ZnO NP（未掺杂的CuO NP）。这些结果表明，纳米氧化物的电子性质可能在所观察到的细胞毒性中起重要作用。这些结果对于选择性地定制毒性效应和建立预测模型以设计具有独特性质的各种类型的纳米材料以及理解纳米材料与生物系统之间的相互作用具有重要意义。(c)2010爱思唯尔有限公司版权所有。
To identify the key physicochemical properties of nano-oxides governing cytotoxicity, we investigate the contribution of the size, shape, morphology, and electronic properties of ten types of insulator (SiO2, CeO2 and Al2O3) and semiconductor (ZnO and CuO) nano-oxides to cytotoxicity using the NIH3T3 and A549 cell lines as models. We find that the shape of the Al2O3 (nanoparticle versus nanowhisker) and the morphology of the SiO2 (porous versus non-porous nanoparticles) did not have obvious effect on the observed cytotoxicity, and the size of the nano-oxides cannot be regarded as an indicator of cytotoxicity. By contrast, we find that the cell viability exposed to the semiconductor nano-oxides was much lower than that exposed to the insulator nano-oxides. Moreover, the Al-doped ZnO nanoparticle (NP) was more toxic than the non-doped ZnO NP, whereas the Al-doped CuO NP was less toxic than the non-doped CuO NP but more toxic than the Al2O3 NP. Correspondingly, the valence band X-ray photoelectron spectra of the nano-oxides show the density of states of the Al-doped ZnO NP (the Al-doped CuO NP) is higher (lower) than that of the non-doped ZnO NP (the non-doped CuO NP). These results suggest that the electronic properties of nano-oxides may play an important role in the observed cytotoxicity. The results have implications for selectively tailoring the toxic effect and establishing predictive models for the design of various types of nanomaterials with unique properties and for the understanding of interactions between nanomaterials with biological system. (c) 2010 Elsevier Ltd. All rights reserved.