Raman-Encoded Molecular Imaging with Topically Applied SERS Nanoparticles for Intraoperative Guidance of Lumpectomy.
Raman-Encoded Molecular Imaging with Topically Applied SERS Nanoparticles for Intraoperative Guidance of Lumpectomy.
复制标题
DOI：
10.1158/0008-5472.can-17-0709
复制
发表时间：
2017-08-15
期刊：
影响因子：
11.2
通讯作者：
Liu JTC
中科院分区：
文献类型：
--
作者：
Wang YW;Reder NP;Kang S;Glaser AK;Yang Q;Wall MA;Javid SH;Dintzis SM;Liu JTC
术中在肿瘤切除术边缘识别癌将能够降低再切除率，目前再切除率高达20- 50%。虽然疾病相关生物标志物的成像可以以高特异性识别恶性肿瘤，但此类生物标志物的多重成像对于以高灵敏度检测分子异质性癌是必要的。我们已经开发了一种拉曼编码的分子成像（REMI）技术，其中靶向纳米粒子局部应用于切除的组织，使手术边缘表面的细胞表面生物标志物的多重面板的快速可视化。进行了首次临床研究，其中使用REMI对57个新鲜标本进行成像，以同时定量四种生物标志物HER 2、ER、EGFR和CD 44的表达。这些生物标志物的联合检测使REMI检测乳腺癌的敏感性达到89.3%，特异性达到92.1%。这些结果突出了REMI检测新鲜切除组织中生物标志物的灵敏度和特异性，这有可能降低癌症患者的再次切除手术率。
Intraoperative identification of carcinoma at lumpectomy margins would enable reduced re-excision rates, which are currently as high as 20–50%. While imaging of disease-associated biomarkers can identify malignancies with high specificity, multiplexed imaging of such biomarkers is necessary to detect molecularly heterogeneous carcinomas with high sensitivity. We have developed a Raman-encoded molecular imaging (REMI) technique in which targeted nanoparticles are topically applied on excised tissues to enable rapid visualization of a multiplexed panel of cell surface biomarkers at surgical margin surfaces. A first-ever clinical study was performed in which 57 fresh specimens were imaged with REMI to simultaneously quantify the expression of four biomarkers HER2, ER, EGFR and CD44. Combined detection of these biomarkers enabled REMI to achieve 89.3% sensitivity and 92.1% specificity for the detection of breast carcinoma. These results highlight the sensitivity and specificity of REMI to detect biomarkers in freshly resected tissue, which has the potential to reduce the rate of re-excision procedures in cancer patients.