DIFFERENTIAL LOCALIZATION OF CELL-SURFACE AND SECRETORY COMPONENTS IN RAT INTESTINAL EPITHELIUM BY USE OF LECTINS
DIFFERENTIAL LOCALIZATION OF CELL-SURFACE AND SECRETORY COMPONENTS IN RAT INTESTINAL EPITHELIUM BY USE OF LECTINS
复制标题
DOI：
10.1083/jcb.62.2.329
复制
发表时间：
1974-01-01
影响因子：
7.8
通讯作者：
BRANSTRATOR, ML
中科院分区：
文献类型：
--
作者：
ETZLER, ME;BRANSTRATOR, ML
用异硫氰酸荧光素标记的凝集素处理成年雄性大鼠小肠的不同层次的切片，这些凝集素分别来自biichos biflorus、lotagonolobus、Ricinus communis和Triticum vulgare（小麦胚芽）。后三种凝集素与幽门附近肠隐窝和绒毛的上皮细胞的微绒毛部分发生反应。这种反应模式在肠的前15厘米处发生了急剧变化，因此在这一点以外的部分，只有隐窝上皮细胞的管腔表面和绒毛底部的绒毛与四角龙杆菌和土豆芽凝集素发生反应，而小麦胚芽凝集素与绒毛内壁细胞的表面发生反应。在小肠远端切片中，所有三种凝集素仅在绒毛底部和隐窝的细胞表面发生反应。这些结果显示了绒毛上不同部位细胞表面成分的差异，以及这些差异对肠道区域的依赖性。雏菊凝集素与大约25%的杯状细胞在不同的肠道水平上发生反应，而与其他三种凝集素的反应性则依赖于肠道的不同区域。
Sections through various levels of small intestine from adult male rats were examined by fluorescence microscopy after treatment with fluorescein isothiocyanate-labeled lectins from Dolichos biflorus, Lotus tetragonolobus, Ricinus communis, and Triticum vulgare (wheat germ). The latter three lectins reacted with the microvillar portion of the epithelial cells lining the crypts and villi in sections of intestine adjacent to the pylorus. This pattern of reactivity was sharply altered along the first 15 cm of intestine so that in sections distal to this point the luminal surfaces of only those epithelial cells in the crypts and at the base of the villi reacted with the L. tetragonolobus and R. communis lectins, whereas the wheat germ lectin reacted with the surfaces of the cells lining the villi. In sections from the distal end of the small intestine, all three lectins reacted with the surfaces of cells only at the base of the villi and in the crypts.These results show a difference in surface components in cells at various portions on the villi and the dependence of these differences on the region of intestine. The D. biflorus lectin-reacted with approximately 25% of the goblet cells at each level of intestine studied whereas the reactivities of the goblet cells with the other three lectins were dependent upon the region of intestine.