SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma.
SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma.
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SLAM 家族成员 8 在间变性大细胞淋巴瘤中表达并增强其生长。
DOI：
10.1038/s41598-020-59530-1
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Haga H.
中科院分区：
文献类型：
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作者：
Sugimoto A;Kataoka TR;Ito H;Kitamura K;Saito N;Hirata M;Ueshima C;Takei Y;Moriyoshi K;Otsuka Y;Nishikori M;Takaori-Kondo A;Haga H.
信号淋巴细胞活化分子家族成员8（SLAMF 8）/巨噬细胞表达的B淋巴细胞活化剂/CD 353是CD 2家族的成员。SLAMF 8抑制巨噬细胞功能，但通过SHP-2促进肿瘤性肥大细胞的生长。在这项研究中，我们发现一些间变性大细胞淋巴瘤（ALCL）样本的SLAMF 8免疫化学阳性。然而，我们发现SLAMF 8阳性和SLAMF 8阴性ALCL样本在年龄、性别、部位或预后方面没有显著差异。我们还鉴定了SLAMF 8在ALCL细胞系Karpas 299和SU-DHL-1中的表达。SLAMF 8基因敲减可降低SHP-2的活化和这些细胞系的生长，并增加这些细胞系的凋亡。此外，我们使用DuoLinkin situkit观察了这些细胞系中SLAMF 8和SHP-2之间的相互作用。总之，这些结果表明SLAMF 8可能通过SHP-2相互作用促进ALCL的生长。
Signaling lymphocytic activation molecule family member 8 (SLAMF8) / B-lymphocyte activator macrophage expressed/CD353 is a member of the CD2 family. SLAMF8 suppresses macrophage function but enhances the growth of neoplastic mast cells via SHP-2. In this study, we found that some anaplastic large cell lymphoma (ALCL) samples were immunohistochemically positive for SLAMF8. However, we found no significant differences between SLAMF8-positive and SLAMF8-negative ALCL samples with respect to age, gender, site, or prognosis. We also identified SLAMF8 expression in ALCL cell lines, Karpas299, and SU-DHL-1. SLAMF8 knockdown decreased the activation of SHP-2 and the growth of these cell lines, and increased the apoptosis of these cell lines. In addition, we observed the interaction between SLAMF8 and SHP-2 in these cell lines using the DuoLinkin situkit. Taken together, these results suggest that SLAMF8 may enhance the growth of ALCL via SHP-2 interaction.