Effects of carbenoxolone on syncytial electrical properties and junction potentials of guinea-pig vas deferens
Effects of carbenoxolone on syncytial electrical properties and junction potentials of guinea-pig vas deferens
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生甲酚对豚鼠输精管合胞体电特性和连接电位的影响
DOI：
10.1007/s00210-006-0109-7
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
R. Manchanda
中科院分区：
文献类型：
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作者：
D. Palani;P. Ghildyal;R. Manchanda
用豚鼠输精管(GPVD)研究了缝隙连接阻滞剂Carbenoxolone对豚鼠输精管(GPVD)平滑肌合胞特性和连接电位的影响。在孵育6-7分钟内，用50μM甘草醚可逆性地增加输入电阻(Rin)(增加682.5 ± 326.0%，P<； 0.05)，并取消线缆电位，而不干扰静止膜电位。Carbenoxolone可逆性地使自发兴奋性连接电位(SEBP)波幅增加96.9 ± 35.45%(P<； 0.05)，使其波幅分布右移，出现频率减少58.17 ± 17.7%(P<； 0.05)，但不改变其时程。同样，甘草酮使兴奋性连接电位的波幅增加17.7 ± 5.88%，使τ降低19.43 ± 8.29%(P<； 0.05)。我们的结果表明，甘草酮通过一种与相对特定的缝隙连接块一致的机制改变了GPVD的电学性质和连接电位。这些结果表明，缝隙连接介导的细胞间通讯可能显著调节GPVD的电特性和连接电位，从而调节该组织的生理功能。
The effects of the putative gap junction blocker carbenoxolone on smooth muscle syncytial properties and junction potentials were studied in guinea pig vas deferens (GPVD). Treatment with 50 μM carbenoxolone reversibly and significantly increased input resistance (Rin) (by 682.5 ± 326.0 %,P< 0.05) and abolished cable potentials within 6–7 mins of incubation, without disturbing resting membrane potential. Carbenoxolone reversibly and significantly increased the amplitude of spontaneous excitatory junction potentials (sEJPs) by 96.9 ± 35.45% (P< 0.05), shifted their amplitude distribution rightwards, and reduced their frequency of occurrence by 58.17 ± 17.7% (P< 0.05), without altering their time courses. Similarly, carbenoxolone increased the amplitude of evoked excitatory junction potentials (eEJPs) by 17.7 ± 5.88% andτdecayby 19.43 ± 8.29% (P< 0.05). Our results indicate that carbenoxolone alters the electrical properties and junctional potentials of the GPVD by a mechanism consistent with a relatively specific block of gap junctions. These results suggest that gap junction mediated cell-to-cell communication may significantly modulate the electrical properties and junctional potentials of the GPVD and consequently the physiological functioning of this tissue.