Two Novel Vesicle-Inducing Proteins in Plastids 1 Genes Cloned and Characterized in Triticum urartu.
Two Novel Vesicle-Inducing Proteins in Plastids 1 Genes Cloned and Characterized in Triticum urartu.
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乌拉尔图质体 1 基因中两种新型囊泡诱导蛋白的克隆和表征
DOI：
10.1371/journal.pone.0170439
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Gao F;Chen B;Jiao J;Jia L;Liu C
VIPP1被认为在逆境胁迫下在类囊体膜的发生和叶绿体被膜的维持中发挥重要作用。Vipp1在光合作用生物中是保守的，并形成一种高度同源低聚物复合体结构，这可能有助于维持叶绿体的膜完整性。本研究克隆了两个新的小麦VIPP1基因，分别命名为TuVipp1和TuVipp2。这两个蛋白与同源蛋白AtVipp1和CrVipp1有很高的同源性。TuVipp1和TuVipp2基因在普通小麦的不同器官中都有表达，这两个基因都受到光和各种胁迫处理的诱导。纯化的TuVipp1和TuVipp2蛋白具有与同源蛋白相似的二级和高级结构。与AtVipp1类似，TuVipp1是叶绿体的靶蛋白。此外，TuVipp1能够挽救AtVipp1(-/-)突变系苍白叶片的表型、致死性和无序的叶绿体结构。综上所述，我们的研究结果表明，TuVipp1和TuVipp2是小麦叶绿体中的功能蛋白，可能在胁迫下维持叶绿体被膜和光合作用后膜生物发生中起关键作用。
Vesicle-inducing protein in plastids 1 (Vipp1) is thought to play an important role both in thylakoid biogenesis and chloroplast envelope maintenance during stress. Vipp1 is conserved in photosynthetic organisms and forms a high homo-oligomer complex structure that may help sustain the membrane integrity of chloroplasts. This study cloned two novel VIPP1 genes from Triticum urartu and named them TuVipp1 and TuVipp2. Both proteins shared high identity with the homologous proteins AtVipp1 and CrVipp1. TuVipp1 and TuVipp2 were expressed in various organs of common wheat, and both genes were induced by light and various stress treatments. Purified TuVipp1 and TuVipp2 proteins showed secondary and advanced structures similar to those of the homologous proteins. Similar to AtVipp1, TuVipp1 is a chloroplast target protein. Additionally, TuVipp1 was able to rescue the phenotypes of pale leaves, lethality, and disordered chloroplast structures of AtVipp1 (-/-) mutant lines. Collectively, our data demonstrate that TuVipp1 and TuVipp2 are functional proteins in chloroplasts in wheat and may be critical for maintaining the chloroplast envelope under stress and membrane biogenesis upon photosynthesis.