Regulatory role and evolution of unconventional NOS-related RNAs
Regulatory role and evolution of unconventional NOS-related RNAs
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DOI：
10.1016/s1872-2423(07)01008-3
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发表时间：
2007-01-01
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影响因子：
--
通讯作者：
O'Shea, Michael
中科院分区：
文献类型：
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作者：
Korneev, Sergei;O'Shea, Michael
一氧化氮合酶（NOS）产生的内源性一氧化氮（NO）在多种生理过程中具有重要作用。然而，一氧化氮的毒性表明其生产必须受到严格监管。 NO 信号传导调节的一个特别令人兴奋和新颖的方面是 NOS 基因的表达可能受到依赖于天然反义转录本 (NAT) 存在的非常规机制的控制。在这里，我们将讨论在池塘蜗牛 Lymnaea stagnalis 的中枢神经系统 (CNS) 中发现的三种不同 NAT 的特性。这些转录本具有与 NOS 编码 mRNA 显着互补的区域。重要的是，我们对已识别的软体动物神经元的实验表明，NAT 通过与 NOS mRNA 形成双链体分子来调节 NOS 基因的表达。最近在哺乳动物中发现的 NOS 相关 NAT 支持我们的软体动物研究结果。因此，NAT 介导的 NO 信号传导调节很可能是多种物种共有的现象。
Endogenous nitic oxide (NO) produced by the enzyme NO synthase (NOS) has an important role in a variety of physiological processes. The toxic properties of NO however suggest that its production must be tightly regulated. A particularly exciting and novel aspect of the regulation of NO signalling is the possibility that the expression of NOS genes is controlled by unconventional mechanisms that depend on the presence of natural antisense transcripts (NATs). Here we will discuss the properties of three distinct NATs discovered in the central nervous system (CNS) of the pond snail Lymnaea stagnalis. These transcripts possess regions of significant complementarity to NOS-encoding mRNAs. Importantly, our experiments on identified molluscan neurons suggest that NATs regulate the expression of the NOS gene through the formation of duplex molecules with the NOS mRNA. Recent discoveries of NOS-related NATs in mammals support the results of our molluscan studies. Thus, it is quite likely that NAT-mediated regulation of NO signalling is a phenomenon shared by a variety of species.